Hosseini Marzieh, Taherkhani Mahboubeh, Ghorbani Nohooji Majid
a Faculty of Pharmaceutical Chemistry, Department of Phytochemistry and Essential Oils Technology, Pharmaceutical Sciences Branch , Islamic Azad University, (IAUPS) , Tehran , Iran.
b Department of Chemistry, College of Science , Takestan Branch , Islamic Azad University , Takestan , Iran.
Nat Prod Res. 2019 Mar;33(6):915-920. doi: 10.1080/14786419.2017.1413573. Epub 2017 Dec 13.
Cardiac glycosides are used for treatment of irregular heartbeats, cardiac arrhythmia and congestive heart failures. In this research, digitoxin as a cardiac glycoside was identified and isolated for the first time in the world from Adonis aestivalis and investigated for its cytotoxic activity against cervical cancer cell (HeLa) lines and human lymphocytes by MTT test. Digitoxin extracted from the aerial parts of the plant collected from west of Iran and purified by column and thin layer chromatographic techniques. The structure of isolated cardiac glycoside was identified by IR, H NMR and C NMR methods and so the presence of digitoxin was established. The half maximal inhibitory concentration values for cervical cancer and lymphocyte cells were obtained to be 5.62 and 412.94 μg/mL. The results of this study introduced the new resource of digitoxin which has considerable cytotoxic effects against HeLa cancer cells but did not damage normal human lymphocyte cells.
强心苷用于治疗心律失常、心脏节律不齐和充血性心力衰竭。在本研究中，首次从夏侧金盏花中鉴定并分离出作为强心苷的洋地黄毒苷，并通过MTT试验研究了其对宫颈癌细胞（HeLa）系和人淋巴细胞的细胞毒性活性。从伊朗西部采集的植物地上部分提取洋地黄毒苷，并通过柱色谱和薄层色谱技术进行纯化。通过红外光谱、氢核磁共振和碳核磁共振方法鉴定了分离出的强心苷的结构，从而确定了洋地黄毒苷的存在。宫颈癌和淋巴细胞的半数最大抑制浓度值分别为5.62和412.94μg/mL。本研究结果介绍了洋地黄毒苷的新来源，其对HeLa癌细胞具有显著的细胞毒性作用，但不会损害正常人类淋巴细胞。
