肝细胞癌中针对CD25衍生肽抗原的循环抗体变化

Change of circulating antibodies against CD25-derived peptide antigen in hepatocellular carcinoma.

作者信息

Wang Jiaxin, Xu Yangchun, Zhao Huan, Zhang Xuan

机构信息

Jilin Provincial Key Laboratory on Molecular and Chemical Genetics, The Second Hospital of Jilin University, Changchun, China.

出版信息

J Cancer Res Ther. 2017;13(5):813-816. doi: 10.4103/jcrt.JCRT_823_17.

DOI:10.4103/jcrt.JCRT_823_17

PMID:29237909

Abstract

AIMS

Several studies have shown altered levels of plasma anti-CD25 antibodies in patients with malignancy in lung, esophagus and breast. The present study was thus designed to test whether circulating anti-CD25 antibody levels were changed in hepatocellular carcinoma (HCC).

METHODS

An enzyme-linked immunosorbent assay (ELISA) was developed in-house to detect plasma IgG antibodies to CD25-derived linear peptide antigens in 122 patients with HCC and 121 control subjects.

RESULTS

Student's t-test showed that plasma anti-CD25 IgG levels were significantly higher in HCC patients than control subjects (t = 4.96, P < 0.001), in which male patients mainly contributed to the increased IgG levels (t = 5.11, P < 0.001). Further analysis showed that plasma anti-CD25 IgG levels were dependent on the stages of HCC although there was no significant correlation between plasma anti-CD25 IgG levels and BCLC stages (r = 0.145, P = 0.110, N = 122); a significant increase in anti-CD25 IgG levels was observed in HCC patients with stages B (t = 4.43, P < 0.001) and C+D (t = 4.89, P < 0.001) as compared with control subjects. Receiver operating characteristic (ROC) curve analysis showed that the area under the ROC curve (AUC) was 0.66 (SE = 0.035, 95% CI 0.60-0.73). The sensitivity of anti-CD25 IgG assay was 12.3% against a specificity of 99.2%.

CONCLUSIONS

The present study suggests that circulating anti-CD25 IgG antibodies may have prognostic rather than early diagnostic values for HCC.

摘要

目的

多项研究表明，肺癌、食管癌和乳腺癌患者血浆抗CD25抗体水平发生改变。因此，本研究旨在检测肝细胞癌（HCC）患者循环抗CD25抗体水平是否发生变化。

方法

自行开发了一种酶联免疫吸附测定（ELISA），用于检测122例HCC患者和121例对照受试者血浆中针对CD25衍生线性肽抗原的IgG抗体。

结果

学生t检验显示，HCC患者血浆抗CD25 IgG水平显著高于对照受试者（t = 4.96，P < 0.001），其中男性患者的IgG水平升高起主要作用（t = 5.11，P < 0.001）。进一步分析表明，尽管血浆抗CD25 IgG水平与BCLC分期之间无显著相关性（r = 0.145，P = 0.110，N = 122），但血浆抗CD25 IgG水平取决于HCC的分期；与对照受试者相比，B期（t = 4.43，P < 0.001）和C + D期（t = 4.89，P < 0.001）的HCC患者抗CD25 IgG水平显著升高。受试者工作特征（ROC）曲线分析显示，ROC曲线下面积（AUC）为0.66（SE = 0.035，95% CI 0.60 - 0.73）。抗CD25 IgG检测的灵敏度为12.3%，特异性为99.2%。