Liu Tong, Song Yan-ni, Shi Qing-Yu, Liu Yang, Bai Xia-nan, Pang Da
Department of Breast Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin, 150040, China.
Tumour Biol. 2014 Apr;35(4):3779-83. doi: 10.1007/s13277-013-1500-x. Epub 2013 Dec 18.
Our recent work suggests that circulating levels of anti-CD25 and anti-FOXP3 antibodies were significantly increased in patients with either lung cancer or esophageal cancer. To confirm if these two autoantibodies are specific for certain types of malignant tumors, the present work was thus undertaken to examine an alteration of anti-CD25 and anti-FOXP3 IgG levels in breast cancer. A total of 152 patients with breast cancer and 112 control subjects were recruited in this study. The levels of circulating anti-CD25 and anti-FOXP3 IgG antibodies were tested using an in-house enzyme-linked immunosorbent assay (ELISA). Student's t test showed no significant differences in the levels of either anti-CD25 IgG or anti-FOXP3 IgG between patients with breast cancer and control subjects, although patients at stage I had increased levels of anti-CD25 IgG compared with control subjects (t = 2.11, P = 0.037); there was no significant association of the anti-FOXP3 IgG levels with stages of breast cancer. In conclusion, circulating IgG autoantibody to CD25 instead of FOXP3 may be a potential biomarker for early diagnosis of breast cancer but further investigation remains needed to replicate this initial finding.
我们最近的研究表明，肺癌或食管癌患者体内抗CD25和抗FOXP3抗体的循环水平显著升高。为了证实这两种自身抗体是否对某些类型的恶性肿瘤具有特异性，因此开展了本研究以检测乳腺癌患者中抗CD25和抗FOXP3 IgG水平的变化。本研究共招募了152例乳腺癌患者和112例对照受试者。使用内部酶联免疫吸附测定（ELISA）检测循环抗CD25和抗FOXP3 IgG抗体的水平。学生t检验显示，乳腺癌患者与对照受试者之间抗CD25 IgG或抗FOXP3 IgG水平均无显著差异，尽管I期患者的抗CD25 IgG水平与对照受试者相比有所升高（t = 2.11，P = 0.037）；抗FOXP3 IgG水平与乳腺癌分期无显著相关性。总之，循环中针对CD25而非FOXP3的IgG自身抗体可能是乳腺癌早期诊断的潜在生物标志物，但仍需进一步研究以重复这一初步发现。
