Liang Jun, Qi Wei-Feng, Xie Shao, Wang Wei-Feng, Zhang Xian-Li, Zhou Xiu-Ping, Hu Jin-Xia, Yu Ru-Tong
Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
Institute of Nervous System Diseases, Lab of Neurosurgery, Xuzhou Medical College, Xuzhou 221002, China.
J Cancer Res Ther. 2017 Oct-Dec;13(6):1032-1037. doi: 10.4103/0973-1482.176176.
WW domain-containing protein 2 (WWP2) is an E3 ubiquitin ligase, which belongs to the NEDD4-like protein family. Recently, it is reported to play a key role in tumorigenesis and development of tumors such as prostate and lung cancer. However, there has been not related report on glioma until now. The aim of this study is to detect the expression of WWP2 and analyze its correlation to the pathological grade and tumor recurrence in patients with glioma.
Western blot and immunohistochemistry were separately used to detect the expression of WWP2 protein in 31 brain glioma tissue samples and 80 brain glioma paraffin specimens. The method of Kaplan-Meier was used to analyze the correlation between the WWP2 expression and glioma recurrence.
The protein expression level of WWP2 in glioma tissue was significantly higher than that in nontumorous brain tissue (P < 0.05), and the protein expression level of WWP2 in high-grade glioma (Grade III-IV) was significantly higher than that in low-grade glioma (Grade I-II) (P < 0.05). Kaplan-Meier analysis indicated that the patients with high WWP2 expression had significantly shorter tumor recurrence time than the patients with low WWP2 expression (P < 0.05).
Our study suggests that WWP2 may play a role in the genesis and development of glioma; it may be a potential biomarker to predict pathological grade and tumor recurrence in patients with glioma.
含WW结构域蛋白2（WWP2）是一种E3泛素连接酶，属于NEDD4样蛋白家族。最近有报道称其在前列腺癌和肺癌等肿瘤的发生发展中起关键作用。然而，迄今为止尚未见有关胶质瘤的相关报道。本研究旨在检测胶质瘤患者中WWP2的表达，并分析其与病理分级及肿瘤复发的相关性。
分别采用蛋白质印迹法和免疫组织化学法检测31例脑胶质瘤组织样本和80例脑胶质瘤石蜡标本中WWP2蛋白的表达。采用Kaplan-Meier法分析WWP2表达与胶质瘤复发的相关性。
胶质瘤组织中WWP2蛋白表达水平显著高于非肿瘤脑组织（P<0.05），高级别胶质瘤（Ⅲ-Ⅳ级）中WWP2蛋白表达水平显著高于低级别胶质瘤（Ⅰ-Ⅱ级）（P<0.05）。Kaplan-Meier分析表明，WWP2高表达患者的肿瘤复发时间显著短于WWP2低表达患者（P<0.05）。
我们的研究提示WWP2可能在胶质瘤的发生发展中起作用；它可能是预测胶质瘤患者病理分级和肿瘤复发的潜在生物标志物。
