Uesaka Karin, Koyama Kenji, Horiuchi Noriyuki, Kobayashi Yoshiyasu, Nishikawa Yoshifumi, Inokuma Hisashi
Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
United Graduate School of Veterinary Sciences, Gifu University, Gifu 501-1193, Japan.
J Vet Med Sci. 2018 Feb 20;80(2):280-283. doi: 10.1292/jvms.17-0205. Epub 2017 Dec 14.
A 4-week-old female Holstein Friesian calf presented with hindlimb paresis. Neurologic examination of spinal reflexes revealed depressed or absent reflexes of the hindlimbs. Menace responses on both sides disappeared on examination of cranial nerves. The calf was finally diagnosed with Neospora caninum infection by pathological findings including nonsuppurative inflammation associated with cysts in the cerebrum and spinal cord. High levels of antibody against recombinant surface antigen 1 of N. caninum (NcSAG1) were detected by ELISA from both serum and cerebrospinal fluid (CSF) samples. This result suggests that detection of antibodies against N. caninum by NcSAG1-ELISA in serum and CSF could be useful for the clinical diagnosis of neosporosis in calves with acquired neurological signs.
一头4周龄的雌性荷斯坦弗里生犊牛出现后肢轻瘫。对脊髓反射进行的神经学检查显示后肢反射减弱或消失。检查颅神经时，两侧的威胁反应消失。通过病理检查发现大脑和脊髓中有与囊肿相关的非化脓性炎症等结果，该犊牛最终被诊断为犬新孢子虫感染。通过酶联免疫吸附测定法（ELISA）从血清和脑脊液（CSF）样本中均检测到了高水平的抗犬新孢子虫重组表面抗原1（NcSAG1）抗体。这一结果表明，通过NcSAG1-ELISA检测血清和脑脊液中抗犬新孢子虫抗体，可能有助于对出现后天神经症状的犊牛进行新孢子虫病的临床诊断。
