Usuki Seigo, Tamura Noriko, Yuyama Kohei, Tamura Tomohiro, Mukai Katsuyuki, Igarashi Yasuyuki
Lipid Biofunction Section, Frontier Research Center for Advanced Material and Life Science, Faculty of Advanced Life Science, Hokkaido University.
National Institute of Advanced Industrial Science and Technology (AIST).
J Oleo Sci. 2018 Jan 1;67(1):77-86. doi: 10.5650/jos.ess17141. Epub 2017 Dec 14.
The tuber of the konjac plant is a source enriched with GlcCer (kGlcCer), and has been used as a dietary supplement to improve the dry skin and itching that are caused by a deficiency of epidermal ceramide. Previously, we showed chemoenzymatically prepared konjac ceramide has a neurite-outgrowth inhibitory effect that is very similar to that of Sema3A and is not seen with animal-type ceramides. While, it has been unclear whether kCer may act on Sema3A or TrkA signaling pathway. In the present study, we showed kCer induces phosphorylation of CRMP2 and microtubules depolymerization via Sema3A signaling pathway not TrkA. It is concluded that kCer may be a potential Sema3A-like agonist that activates Sema3A signaling pathway directly.
魔芋植物的块茎是富含葡糖神经酰胺（kGlcCer）的来源，并已被用作膳食补充剂，以改善因表皮神经酰胺缺乏引起的皮肤干燥和瘙痒。此前，我们发现化学酶法制备的魔芋神经酰胺具有与Sema3A非常相似的神经突生长抑制作用，而动物型神经酰胺则没有这种作用。然而，目前尚不清楚kCer是否可能作用于Sema3A或TrkA信号通路。在本研究中，我们发现kCer通过Sema3A信号通路而非TrkA诱导CRMP2磷酸化和微管解聚。结论是，kCer可能是一种潜在的类Sema3A激动剂，可直接激活Sema3A信号通路。
