Lein Pamela, Higgins Dennis
Department of Biology, Canisius College, Buffalo, NY 14208.
Department of Pharmacology, School of Medicine, State University of New York, Buffalo, NY 14214, USA.
Biomed Res (Aligarh). 1996;7(1):101-111.
We have examined the role of integrins in the development of dendrites in embryonic rat sympathetic neurons . Previous studies have established that exposure to a basement membrane extract (BME) causes these neurons to form dendrites (Lein and Higgins, 1989). The current experiments demonstrate that the dendrite-promoting activity of BME can be markedly inhibited by antibodies directed against the integrin subunit. Although the specific ∝ integrin sub-unit(s) that mediate the effect of BME on dendritic growth were not identified, it was found that neither the ∝, or ∝ sub-units, nor RGD-binding integrins were involved to any significant extent. These data suggest that, in addition to their well established role in regulating axonal growth, integrins also participate in dendritic growth.
我们研究了整合素在胚胎大鼠交感神经元树突发育中的作用。先前的研究已证实，暴露于基底膜提取物（BME）会使这些神经元形成树突（莱因和希金斯，1989年）。当前实验表明，针对整合素亚基的抗体可显著抑制BME的树突促进活性。尽管尚未确定介导BME对树突生长作用的特定α整合素亚基，但发现α1或α2亚基以及RGD结合整合素均未在很大程度上参与其中。这些数据表明，整合素除了在调节轴突生长中已确立的作用外，还参与树突生长。
