Kim Ik-Hyun, Han Jae-Yeong, Cho In-Sook, Ju HyeKyoung, Moon Jae Sun, Seo Eun-Young, Kim Hong Gi, Hammond John, Lim Hyoun-Sub
Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea.
Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Wanju 55365, Korea.
Plant Pathol J. 2017 Dec;33(6):608-613. doi: 10.5423/PPJ.NT.05.2017.0106. Epub 2017 Dec 1.
The full-length sequence of a new isolate of (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned into a binary vector downstream of the bacteriophage T7 RNA promoter and the 35S promoter. was successfully infected using transcripts synthesized using the T7 promoter, detected at 20 days post inoculation (dpi), but did not produce obvious symptoms. and were inoculated through agroinfiltration. At 32 dpi the infection rate was evaluated; no plants were infected by agroinfiltration, but infection of was obtained.
来自韩国的苹果褪绿叶斑病毒（ACLSV）一个新分离株的全长序列存在差异，但与日本分离株A4关系最为密切，核苷酸同一性为84%。将韩国ACLSV分离株的全长cDNA克隆到噬菌体T7 RNA启动子和花椰菜花叶病毒35S启动子下游的二元载体中。使用T7启动子合成的转录本成功感染了该病毒，在接种后20天（dpi）检测到，但未产生明显症状。通过农杆菌浸润接种了本氏烟草和三生烟草。在32 dpi时评估感染率；通过农杆菌浸润没有感染本氏烟草植株，但获得了三生烟草的感染。
