Phytopathology. 2005 Apr;95(4):420-6. doi: 10.1094/PHYTO-95-0420.
ABSTRACT A trichovirus closely related to Apple chlorotic leaf spot virus (ACLSV) was detected in symptomatic apricot and Japanese plum from Italy. The Sus2 isolate of this agent cross-reacted with anti-ACLSV polyclonal reagents but was not detected by broad-specificity anti- ACLSV monoclonal antibodies. It had particles with typical trichovirus morphology but, contrary to ACLSV, was unable to infect Chenopodium quinoa and C. amaranticolor. The sequence of its genome (7,494 nucleotides [nt], missing only approximately 30 to 40 nt of the 5' terminal sequence) and the partial sequence of another isolate were determined. The new virus has a genomic organization similar to that of ACLSV, with three open reading frames coding for a replication-associated protein (RNA-dependent RNA polymerase), a movement protein, and a capsid protein, respectively. However, it had only approximately 65 to 67% nucleotide identity with sequenced isolates of ACLSV. The differences in serology, host range, genome sequence, and phylogenetic reconstructions for all viral proteins support the idea that this agent should be considered a new virus, for which the name Apricot pseudo-chlorotic leaf spot virus (APCLSV) is proposed. APCLSV shows substantial sequence variability and has been recovered from various Prunus sources coming from seven countries, an indication that it is likely to have a wide geographical distribution.
摘要 从意大利的症状性杏仁和日本李中检测到与苹果褪绿斑病毒（ACLSV）密切相关的毛毡病毒。该制剂的 Sus2 分离株与抗 ACLSV 多克隆试剂发生交叉反应，但未被广谱抗 ACLSV 单克隆抗体检测到。它具有典型的毛毡病毒形态的颗粒，但与 ACLSV 不同，它无法感染藜麦和苋色藜。确定了其基因组（7494 个核苷酸[nt]，仅缺失大约 5'末端序列的 30 至 40 个 nt）和另一个分离株的部分序列。新病毒的基因组组织与 ACLSV 相似，分别编码复制相关蛋白（RNA 依赖性 RNA 聚合酶）、运动蛋白和衣壳蛋白的三个开放阅读框。然而，它与已测序的 ACLSV 分离株的核苷酸同一性约为 65%至 67%。血清学、宿主范围、基因组序列和所有病毒蛋白的系统发育重建方面的差异支持这样一种观点，即该制剂应被视为一种新病毒，建议将其命名为杏伪褪绿斑病毒（APCLSV）。APCLSV 显示出大量的序列变异性，并从来自七个国家的各种李属来源中回收，表明它可能具有广泛的地理分布。
