Lenz Ryan R, Dai Wenhao
Department of Plant Sciences, North Dakota State University, Fargo, ND, United States.
Front Plant Sci. 2017 Nov 29;8:2057. doi: 10.3389/fpls.2017.02057. eCollection 2017.
Phytoplasmas such as " Phytoplasma pruni," the causal agent of X-disease of stone fruits, lack detailed biological analysis. This has limited the understanding of plant resistance mechanisms. Chokecherry ( L.) is a promising model to be used for the plant-phytoplasma interaction due to its documented ability to resist X-disease infection. A consensus chokecherry genetic map "Cho" was developed with JoinMap 4.0 by joining two parental maps. The new map contains a complete set of 16 linkage groups, spanning a genetic distance of 2,172 cM with an average marker density of 3.97 cM. Three significant quantitative trait loci (QTL) associated with X-disease resistance were identified contributing to a total of 45.9% of the phenotypic variation. This updated genetic linkage map and the identified QTL will provide the framework needed to facilitate molecular genetics, genomics, breeding, and biotechnology research concerning X-disease in chokecherry and other species.
诸如“李属植原体”（核果类X病的病原体）之类的植原体缺乏详细的生物学分析。这限制了对植物抗性机制的理解。由于其已被证明具有抵抗X病感染的能力，苦樱桃（Prunus virginiana L.）是用于植物与植原体相互作用研究的一个很有前景的模式植物。利用JoinMap 4.0通过合并两个亲本图谱构建了一张苦樱桃共识遗传图谱“Cho”。新图谱包含一整套16个连锁群，跨越2172厘摩的遗传距离，平均标记密度为3.97厘摩。鉴定出了3个与X病抗性相关的显著数量性状位点（QTL），它们总共贡献了45.9%的表型变异。这张更新的遗传连锁图谱以及鉴定出的QTL将为促进有关苦樱桃和其他物种中X病的分子遗传学、基因组学、育种及生物技术研究提供所需的框架。
