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包含[具体对象]表面抗原的多表位抗原的克隆、表达及纯化

Cloning, expression and purification of a polytopic antigen comprising of surface antigens of .

作者信息

Alibakhshi Abbas, Bandehpour Mojgan, Kazemi Bahram

机构信息

Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Iran J Microbiol. 2017 Aug;9(4):251-256.

PMID:29238461
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5723978/
Abstract

BACKGROUND AND OBJECTIVES

Polytopic antigens are recently applied for replacing crude antigens, for control of infectious agents. The surface of the Toxoplasma is covered with immunogenic antigens namely surface antigens (SAGs). These antigens possess several immunogenic epitopes, inducing immune responses.

MATERIALS AND METHODS

In this study, a DNA construct comprising of sequences encoding epitopes from SAG1, 2 and 3 was designed and cloned into pET28a expression vector and subsequently expressed and purified, using Ni-NTA column.

RESULTS

The SDS-PAGE and Western blotting analysis showed that polytopic genes were successfully expressed and purified.

CONCLUSION

The surface antigenic protein of can be applied in the future epitope-based applications.

摘要

背景与目的

多表位抗原最近被用于替代粗抗原，以控制感染因子。弓形虫表面覆盖有免疫原性抗原，即表面抗原（SAGs）。这些抗原拥有多个免疫原性表位，可诱导免疫反应。

材料与方法

在本研究中，设计了一种包含编码SAG1、2和3表位序列的DNA构建体，并将其克隆到pET28a表达载体中，随后使用镍-亚氨基二乙酸（Ni-NTA）柱进行表达和纯化。

结果

十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质免疫印迹分析表明，多表位基因已成功表达并纯化。

结论

[此处原文缺失具体物种]的表面抗原蛋白可用于未来基于表位的应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f9/5723978/eef991cfcc88/IJM-9-251-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f9/5723978/1c4d0fe12b3b/IJM-9-251-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f9/5723978/4a82df606735/IJM-9-251-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f9/5723978/13b03feca9e5/IJM-9-251-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f9/5723978/eef991cfcc88/IJM-9-251-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f9/5723978/1c4d0fe12b3b/IJM-9-251-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f9/5723978/4a82df606735/IJM-9-251-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f9/5723978/13b03feca9e5/IJM-9-251-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f9/5723978/eef991cfcc88/IJM-9-251-g004.jpg

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