Daghigh Faeze, Alihemmati Alireza, Karimi Pouran, Habibi Parisa, Ahmadiasl Naser
Tuberculosis and Lung Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Histology & Embryology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran J Basic Med Sci. 2017 Dec;20(12):1312-1317. doi: 10.22038/IJBMS.2017.9599.
The role of isoflavones in pulmonary structure and function during menopause is not well studied. Moreover, the important role of estrogen in the physiological function of respiratory system has been revealed. Genistein, as an isoflavone, mimics estrogenic in diabetic and ovariectomized rats. Here, we hypothesized that genistein would reverse changes in the protein expression levels related to estrogen deficiency in the lung of ovariectomized diabetic rats.
Wistar female rats were assigned to four experimental groups (n=10 in each group): sham, rats underwent laparotomy without removing the ovaries; OVX, rats that underwent ovariectomy; OVX.D, rats underwent bilateral ovariectomy and were fed a high-fat diet (HFD); OVX.D.G, ovariectomized diabetic rats with genistein administration (1 mg/kg /day). After ovariectomy, rats continued to feed HFD for a 4-week period. After 4 weeks of HFD feeding, a single dose of 30 mg/kg of streptozotocin was administered in the diabetic group. Genistein was administered for eight weeks. At the end of the experiment, lung tissue was removed and Western blotting technique and hematoxylin-eosin staining were used for evaluation of the lung.
Treatment with genistein significantly decreased inflammatory and apoptotic biomarkers in the ovariectomized diabetic rats compared to non-treated animals (<0.05). Also, genistein exerted a protective effect in the lung architecture.
Genistein partly reversed ovariectomy-induced changes in apoptotic and inflammatory biomarkers in the lung. Our data suggest that genistein treatment as a natural replacement therapy may prevent the estrogen deficiency effects in the lung of diabetic menopausal women.
异黄酮在绝经期间对肺结构和功能的作用尚未得到充分研究。此外，雌激素在呼吸系统生理功能中的重要作用已被揭示。染料木黄酮作为一种异黄酮，在糖尿病和去卵巢大鼠中具有雌激素样作用。在此，我们假设染料木黄酮可逆转去卵巢糖尿病大鼠肺中与雌激素缺乏相关的蛋白质表达水平变化。
将Wistar雌性大鼠分为四个实验组（每组n = 10）：假手术组，即接受剖腹手术但未切除卵巢的大鼠；去卵巢组，即接受卵巢切除术的大鼠；OVX.D组，即接受双侧卵巢切除术并喂食高脂饮食（HFD）的大鼠；OVX.D.G组，即给予染料木黄酮（1 mg/kg/天）的去卵巢糖尿病大鼠。卵巢切除术后，大鼠继续喂食HFD 4周。在喂食HFD 4周后，糖尿病组单次给予30 mg/kg链脲佐菌素。给予染料木黄酮8周。实验结束时，取出肺组织，采用蛋白质印迹技术和苏木精-伊红染色对肺进行评估。
与未治疗的动物相比，染料木黄酮治疗显著降低了去卵巢糖尿病大鼠的炎症和凋亡生物标志物水平（<0.05）。此外，染料木黄酮对肺结构具有保护作用。
染料木黄酮部分逆转了去卵巢诱导的肺中凋亡和炎症生物标志物的变化。我们的数据表明，染料木黄酮治疗作为一种天然替代疗法，可能预防糖尿病绝经后女性肺部的雌激素缺乏效应。
