Department of Biosciences, COMSATS Institute of Information Technology, Park Road, Islamabad, Pakistan.
National University of Medical Sciences (NUMS), The Mall, Rawalpindi, Pakistan.
Eur J Clin Microbiol Infect Dis. 2018 Apr;37(4):691-700. doi: 10.1007/s10096-017-3161-y. Epub 2017 Dec 14.
The prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Pakistan is known to be high, but very few studies have described the molecular epidemiology of the different MRSA clones circulating in the country. Forty-four MRSA isolates were collected from two tertiary care hospitals of the Rawalpindi district of Pakistan. All strains were identified by a conventional phenotypic method and then subjected to genotyping by microarray hybridisation. Six clonal complexes (CCs) and 19 strains were identified. The most commonly identified strains were: (i) Panton-Valentine leucocidin (PVL)-positive CC772-MRSA-V, "Bengal Bay Clone" (ten isolates; 22.3%), (ii) ST239-MRSA [III + ccrC] (five isolates) and (iii) a CC8-MRSA-IV strain, as well as CC6-MRSA-IV (both with four isolates; 9.1% each). Several of the strains detected indicated epidemiological links to the Middle Eastern/Arabian Gulf region. Further studies are needed to type MRSA from countries with less known epidemiology and to monitor the distribution and spread of strains, as well as possible links to global travel, migration and commerce.
耐甲氧西林金黄色葡萄球菌（MRSA）在巴基斯坦的流行率已知很高，但很少有研究描述过该国流行的不同 MRSA 克隆的分子流行病学。从巴基斯坦拉瓦尔品第地区的两家三级护理医院收集了 44 株 MRSA 分离株。所有菌株均通过传统表型方法鉴定，然后通过微阵列杂交进行基因分型。鉴定出了 6 个克隆复合体（CC）和 19 株菌株。最常见的菌株包括：（i）产 Panton-Valentine 白细胞素（PVL）的 CC772-MRSA-V“孟加拉湾克隆”（10 株；22.3%），（ii）ST239-MRSA [III + ccrC]（5 株）和（iii）CC8-MRSA-IV 菌株，以及 CC6-MRSA-IV（各 4 株；9.1%）。检测到的一些菌株表明与中东/阿拉伯海湾地区存在流行病学联系。需要进一步研究来自流行病学知识较少的国家的 MRSA 分型，以监测菌株的分布和传播，以及与全球旅行、移民和商业的可能联系。
