Ohadian Moghadam Solmaz, Pourmand Mohammad Reza, Mahmoudi Mahmood, Sadighian Hooman
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran 1417653761, Iran.
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran 1417653761, Iran
FEMS Microbiol Lett. 2015 Apr;362(8):fnv043. doi: 10.1093/femsle/fnv043. Epub 2015 Mar 20.
Information about the molecular structure of MRSA strains provides significant insights into the epidemiology of this important pathogen. To investigate the molecular characteristics of MRSA isolates, MRSA isolates were subjected to molecular typing by means of spa typing, multilocus sequence typing, Staphylococcal Cassette Chromosome mec (SCCmec) grouping and to phenotypic antimicrobial susceptibility testing by means of disk diffusion assay. Then the presence of pvl genes was evaluated. Cluster analysis by eBURSTv3 showed that MRSA isolates belonged to two major clonal complexes (CC); CC8 (ST239, ST585, ST2732, ST1294) and CC30 (ST30, ST36, ST1163) and four singletons. Subsequent analysis of MRSA isolates revealed that the most prevalent SCCmec type was type III (55.8%) followed by type IV (34.9%) and type II (2.3%). Totally 11 different spa types were discriminated among which types t037 and t030 were predominant. The prevalence of Panton-Valentine leukocidin (PVL)-positive MRSA strains was high (20%), which is a matter of great concern, because the PVL is frequently associated with severe and recurrent SSTIs. ST239-III- t037 represented the most predominant MRSA clone. The presence of sequence type (ST) 36 and ST 121 are being reported for the first time in Iran.
有关耐甲氧西林金黄色葡萄球菌（MRSA）菌株分子结构的信息为深入了解这种重要病原体的流行病学提供了重要线索。为了研究MRSA分离株的分子特征，采用spa分型、多位点序列分型、葡萄球菌盒式染色体mec（SCCmec）分型等方法对MRSA分离株进行分子分型，并通过纸片扩散法进行表型抗菌药敏试验。然后评估pvl基因的存在情况。通过eBURSTv3进行的聚类分析表明，MRSA分离株属于两个主要的克隆复合体（CC）；CC8（ST239、ST585、ST2732、ST1294）和CC30（ST30、ST36、ST1163）以及四个单例。对MRSA分离株的后续分析显示，最常见的SCCmec类型是III型（55.8%），其次是IV型（34.9%）和II型（2.3%）。共鉴别出11种不同的spa类型，其中t037和t030类型占主导。泛素-溶素（PVL）阳性的MRSA菌株患病率很高（20%），这是一个令人高度关注的问题，因为PVL常与严重和复发性皮肤和软组织感染（SSTIs）相关。ST239-III-t037代表最主要的MRSA克隆。序列类型（ST）36和ST121在伊朗首次被报道。
