Siatkin S P, Frolov V A
Biull Eksp Biol Med. 1989 Feb;107(2):184-6.
Rapid spectrophotometric method of lysosome stability determination depending on hydrogen ion concentration is described. The time of analysis is decreased by 5-6 h in comparison with enzymic method. The process of lysosome degradation was linear at pH 6. The incubation mixture acidity dependence curve of lysosome lysis extend was complex. The lysosome lysis rate rapidly increased at pH much less than 6 less than pH. Lysosome incubation at 0-4 degrees C during 24 h decreased its sensitivity to incubation mixture acidity within the whole investigated pH range. Isolated lysosome acid resistance may be used as an index of its stability and lability in vivo and in vitro by various physicochemical factors. Percentage of initial absorbtion (A520) and initial lysosome lysis rate (delta A520/min) may be index of such effect.
描述了一种基于氢离子浓度的快速分光光度法测定溶酶体稳定性。与酶法相比，分析时间减少了5 - 6小时。溶酶体降解过程在pH 6时呈线性。溶酶体裂解程度的孵育混合物酸度依赖性曲线很复杂。在pH远低于6时，溶酶体裂解速率迅速增加。溶酶体在0 - 4℃下孵育24小时会降低其在整个研究pH范围内对孵育混合物酸度的敏感性。分离的溶酶体耐酸性可作为其在体内和体外受各种物理化学因素影响的稳定性和不稳定性指标。初始吸光度（A520）百分比和初始溶酶体裂解速率（ΔA520/分钟）可作为这种效应的指标。
