Frolov V A, Siatkin S P
Vopr Med Khim. 1990 Nov-Dec;36(6):36-9.
Physico-chemical properties of intact lysosomes isolated from rat liver tissue were studied using spectrophotometric methods. The lysosomal suspension did not show specific maximal peaks in spectra of light passing through, scattering and absorbance. Stability of the suspension was most suitably evaluated at 520 nm. The rate of lysosomes lysis was distinctly increased at temperature above 37 degrees at pH below 3.0 and above 7.0 as well as in osmotic pressure (pi) exceeding 0.75 MPa. The temperature of phase conversion at 12.5 degrees and 38.5 degrees was calculated from Arrhenius plot. Activating energy of the lysosomes thermolysis was equal to 50, 2 and 18 kcal/mol for solid, mixed and liquid phases of lysosomal membranes, respectively. Additional incubation of the initial lysosomal suspension at 0-4 degrees within 24 hrs led to an increase in osmosis- and acid-stability within all the ranges of pi and pH values studies as well as to decrease of its sensitivity to hypothermia (10-30 degrees) but not to hyperthermia (above 37 degrees). Cadaverine at final concentration 1.0 and 0.1 mM protected lysosomes from thermolysis. Osmotic-resistance, acid-stability and thermotolerance may be used as available dynamic parameters of the particles structure state as well as in evaluation of deteriorating effects of various physicochemical factors in vivo and in vitro.
采用分光光度法研究了从大鼠肝脏组织中分离出的完整溶酶体的物理化学性质。溶酶体悬浮液在透光、散射和吸光光谱中未显示出特定的最大峰。悬浮液的稳定性在520nm处评估最为合适。在pH低于3.0和高于7.0以及渗透压（π）超过0.75MPa的情况下，当温度高于37℃时，溶酶体的裂解速率明显增加。根据阿伦尼乌斯图计算出在12.5℃和38.5℃时的相变温度。溶酶体膜的固相、混合相和液相热解的活化能分别等于50、2和18kcal/mol。将初始溶酶体悬浮液在0-4℃下额外孵育24小时，导致在所有研究的π和pH值范围内，其渗透稳定性和酸稳定性增加，同时对低温（10-30℃）的敏感性降低，但对高温（高于37℃）的敏感性未降低。终浓度为1.0和0.1mM的尸胺可保护溶酶体免受热解。渗透抗性、酸稳定性和耐热性可用作颗粒结构状态的有效动态参数，以及用于评估体内外各种物理化学因素的恶化作用。
