评估一种使用唾液样本进行牙周炎临床诊断的多重靶向蛋白质组学方法。
Assessing a multiplex-targeted proteomics approach for the clinical diagnosis of periodontitis using saliva samples.
作者信息
Mertens Brenda, Orti Valérie, Vialaret Jérôme, Gibert Philippe, Relaño-Ginés Aroa, Lehmann Sylvain, Deville de Périère Dominique, Hirtz Christophe
机构信息
U.F.R. d'Odontologie, Département de Parodontologie, 545, avenue du Professeur Jean-Louis Viala, 34 193 Montpellier, Cedex 5.
University of Montpellier, LBPC - IRMB, CHU de Montpellier, 80 rue Augustin Fliche, 34 295 Montpellier, France.
出版信息
Bioanalysis. 2018 Jan;10(1):35-45. doi: 10.4155/bio-2017-0218. Epub 2017 Dec 15.
AIM
The present study focused on the research of new biomarkers based on the liquid chromatography-multiple-reaction monitoring (MRM) proteomic profile in whole saliva of patients with periodontitis compared with periodontal healthy patients.
METHODS
A 30-min multiplexed liquid chromatography-MRM method was used for absolute quantification of 35 plasma biomarkers in saliva from control patients and patients with periodontitis.
RESULTS
Three proteins namely hemopexin, plasminogen and α-fibrinogen were shown to be clearly related to the presence of periodontitis compared with healthy patients. Apolipoprotein H was found to discriminate for the first time chronic and aggressive periodontitis.
CONCLUSION
Our results indicate that this innovative MRM method could be used to screen for periodontitis in clinical environment. Furthermore, apolipoprotein H was found to be a discriminant biomarker of aggressive periodontitis.
目的
本研究聚焦于基于液相色谱-多反应监测（MRM）蛋白质组学分析，对牙周炎患者与牙周健康患者全唾液中的新型生物标志物进行研究。
方法
采用30分钟多重液相色谱-MRM方法对对照患者和牙周炎患者唾液中的35种血浆生物标志物进行绝对定量分析。
结果
与健康患者相比，血色素结合蛋白、纤溶酶原和α-纤维蛋白原这三种蛋白质与牙周炎的存在明显相关。载脂蛋白H首次被发现可区分慢性和侵袭性牙周炎。
结论
我们的结果表明，这种创新的MRM方法可用于临床环境中牙周炎的筛查。此外，载脂蛋白H被发现是侵袭性牙周炎的鉴别生物标志物。
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