Bcl-2 和 Bax 在腮腺萎缩中的作用。

Role of Bcl-2 and Bax in parotid gland atrophy.

机构信息

Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Binzhou Medical College, Binzhou, China.

出版信息

Eur Rev Med Pharmacol Sci. 2017 Dec;21(23):5315-5320. doi: 10.26355/eurrev_201712_13914.

DOI:10.26355/eurrev_201712_13914

PMID:29243771

Abstract

OBJECTIVE

To investigate the expression and correlation of B-cell lymphoma-2 (Bcl-2) and Bax in the parotid gland after leading duct ligation in rat.

MATERIALS AND METHODS

Atrophy of the right parotid was induced by ligating the right Stensen's duct of rats. Immunohistochemical labeling was performed to study the changes in number and distribution of Bcl-2 and Bax in each step of glandular atrophy, and every group at 1, 3, 5, 7, 14, 21, 30, 60, 90, 150, 180 days after ligation.

RESULTS

Bcl-2 and Bax showed a low level of expression in normal glandular tissues. At different time points after the ligation of the main duct, Bcl-2 was highly expressed in the duct cells, and the absorbance value reached a peak value at 21-day (3.02+0.10). The 1 D expression of Bax was found in some of the cells in the 3 D, and the expression of Bax reached the peak (1.99+0.10), and the expression of Bcl-2 and Bax were decreased in some cells. Bcl-2/Bax ratio increased at 1 day-21 day, and then decreased and stabilized.

CONCLUSIONS

The expression of Bax and Bcl-2 after ligation of the parotid gland is closely related to the process of the parotid gland atrophy.

摘要

目的

研究在大鼠主导管结扎后，B 细胞淋巴瘤-2（Bcl-2）和 Bax 在腮腺中的表达及相关性。

材料与方法

通过结扎大鼠右侧腮腺主导管诱导右侧腮腺萎缩。采用免疫组织化学标记法研究在腺体萎缩的各个阶段 Bcl-2 和 Bax 的数量和分布变化，以及结扎后第 1、3、5、7、14、21、30、60、90、150、180 天的每一组的变化。

结果

Bcl-2 和 Bax 在正常腺体组织中表达水平较低。在主导管结扎后的不同时间点，Bcl-2 在导管细胞中高表达，吸光度值在 21 天达到峰值（3.02+0.10）。Bax 在第 3 天的一些细胞中发现了 1D 表达，Bax 的表达达到峰值（1.99+0.10），而一些细胞中 Bcl-2 和 Bax 的表达减少。Bcl-2/Bax 比值在第 1 天至第 21 天增加，然后减少并稳定。