海鳗（Conger myriaster）皮肤黏液中一种半乳糖结合凝集素的纯化与表征

Purification and characterization of a galactose-binding lectin from the skin mucus of the conger eel Conger myriaster.

Shiomi K, Uematsu H, Yamanaka H, Kikuchi T

Department of Food Science and Technology, Tokyo University of Fisheries, Japan.

Comp Biochem Physiol B. 1989;92(2):255-61. doi: 10.1016/0305-0491(89)90275-7.

Abstract

A galactose-binding lectin was purified from the skin mucus of the conger eel Conger myriaster by affinity chromatography and HPLC. 2. The lectin was a simple protein having the same two subunits with a mol. wt of 12,500 and a N-terminal amino acid of phenylalanine. 3. Electrofocusing suggested that the purified lectin was composed of several isolectins. 4. From the ultraviolet difference spectra attributable to tryptophanyl residues in the binding site, the binding constant of the lectin for D-galactose was estimated to be 5.3 x 10(3)/M.

摘要

通过亲和色谱法和高效液相色谱法从星康吉鳗的皮肤黏液中纯化出一种半乳糖结合凝集素。2. 该凝集素是一种简单蛋白质，由两个相同的亚基组成，分子量为12,500，N端氨基酸为苯丙氨酸。3. 等电聚焦表明纯化的凝集素由几种同工凝集素组成。4. 根据结合位点中色氨酸残基的紫外差光谱，估计该凝集素对D-半乳糖的结合常数为5.3×10³/M。