黑指纹海兔（Aplysia kurodai）皮肤中一种D-半乳糖结合凝集素的纯化及其细胞附着活性

Purification and cell attachment activity of a D-galactose-binding lectin from the skin of sea hare, Aplysia kurodai.

作者信息

Ozeki Y

机构信息

Department of System Element, Faculty of Science, Yokohama City University, Yokohama, Japan.

出版信息

Biochem Mol Biol Int. 1998 Aug;45(5):989-95. doi: 10.1002/iub.7510450516.

DOI:10.1002/iub.7510450516

PMID:9739463

Abstract

A D-galactose-binding lectin which does not require Ca2+ or reducing reagents to induce activity was purified from skin of sea hare, Aplysia kurodai, by affinity chromatography. Skin lectin was confirmed to be a disulfide-bonded heteromultimer with molecular mass of 200 kDa, consisting of 28 kDa and 26 kDa subunits by SDS-PAGE and two-dimensional SDS-PAGE. Human rhabdomyosarcoma cells attached to and spread on plastic plates coated with lectin. Cell adhesion induced by lectin was completely inhibited by the addition of lactose.

摘要

通过亲和层析从黑指纹海兔（Aplysia kurodai）的皮肤中纯化出一种D-半乳糖结合凝集素，该凝集素诱导活性不需要Ca2+或还原试剂。通过SDS-PAGE和二维SDS-PAGE证实皮肤凝集素是一种分子量为200 kDa的二硫键结合异源多聚体，由28 kDa和26 kDa的亚基组成。人横纹肌肉瘤细胞附着在涂有凝集素的塑料板上并在其上铺展。添加乳糖可完全抑制凝集素诱导的细胞黏附。