Institute of Chemical Biology and Fundamental Medicine, Novosibirsk, Russia.
Novosibirsk State University, Novosibirsk, Russia.
Immunol Res. 2018 Feb;66(1):141-150. doi: 10.1007/s12026-017-8981-4.
Inflammation was shown to be activated in varicose veins, although its role in the development of vein wall transformation remains inconclusive. We aimed to investigate the influence of 13 inflammation-related single nucleotide polymorphisms (SNPs) TNF rs1800629 and rs3093661, IL1A rs1800587, IL1RN rs4251961, IL6 rs1800795 and rs1800796, IFNG rs2430561, IL10 rs1800896, TGFB1 rs1800469, HIF1A rs11549465, NFKB1 rs28362491, and rs4648068 on the risk of primary varicose veins (PVVs) in ethnic Russians. We genotyped 709 patients with PVVs and 278 individuals without a history of chronic venous disease and performed a single SNP and a haplotype analysis. Several associations with P < 0.05 were revealed in our study. Variant allele HIF1A rs11549465 T, TNF rs3093661 A, and NFKB1 rs28362491 ATTG deletion showed the reverse association with PVV risk, and allele IL6 rs1800795 C was associated with the increased risk of the studied pathology. Haplotype analysis revealed associations of TNF haplotypes rs3093661 A-rs1800629 G and IL6 rs1800795 C-rs1800796 G with the decreased and the increased risk of PVVs, correspondingly. However, all the observed associations failed to reach statistical significance after the correction for multiple testing, which was set at a level of 10 due to many tests performed. Our study therefore provides evidence that investigated polymorphisms do not play a major role in susceptibility to PVVs.
炎症被证实存在于静脉曲张中，但其在静脉壁病变发展中的作用仍不确定。我们旨在研究 13 个炎症相关的单核苷酸多态性（SNP）TNF rs1800629 和 rs3093661、IL1A rs1800587、IL1RN rs4251961、IL6 rs1800795 和 rs1800796、IFNG rs2430561、IL10 rs1800896、TGFB1 rs1800469、HIF1A rs11549465、NFKB1 rs28362491 和 rs4648068 在俄罗斯人群中原发性静脉曲张（PVVs）风险中的影响。我们对 709 例 PVVs 患者和 278 例无慢性静脉疾病病史的个体进行了基因分型，并进行了单 SNP 和单倍型分析。在我们的研究中发现了几个与 P<0.05 相关的关联。变异等位基因 HIF1A rs11549465 T、TNF rs3093661 A 和 NFKB1 rs28362491 ATTG 缺失与 PVV 风险呈反向关联，等位基因 IL6 rs1800795 C 与研究病理的风险增加相关。单倍型分析显示 TNF 单倍型 rs3093661 A-rs1800629 G 和 IL6 rs1800795 C-rs1800796 G 与 PVVs 风险降低和增加相关。然而，由于进行了多次测试，在对多重测试进行校正后，所有观察到的关联均未达到统计学意义，我们的研究因此提供了证据表明，所研究的多态性在 PVVs 的易感性中不起主要作用。
