Vrije Universiteit Brussel (VUB), Universitair Ziekenhuis Brussel (UZB), Department of Microbiology and Infection Control, Laarbeeklaan 101, 1090, Brussels, Belgium.
Eur J Clin Microbiol Infect Dis. 2018 Apr;37(4):711-714. doi: 10.1007/s10096-017-3163-9. Epub 2017 Dec 16.
The purpose of this study was to test a newly developed decontamination and fluidization kit for processing respiratory specimens for the detection of mycobacteria: the Myco-TB procedure (developed by Copan (Brescia, Italy)). This technique was compared with the Zephiran decontamination method in use in our hospital. Respiratory specimens (n = 387: 130 endotracheal/bronchial aspirates, 172 bronchoalveolar lavages and 55 sputa) submitted to the University Hospital of Brussels between January 2016 and March 2017 were included. All samples were divided into two aliquots: one was subjected to the Myco-TB method and one to the Zephiran technique prior to culture. The sensitivities for culture for the Zephiran technique on solid media, the Myco-TB method on solid media and Myco-TB combined with the MGIT™ system were respectively 67%, 87% and 89%. The contamination rates were 22% with both the Zephiran and Myco-TB method on solid media and only 4% with the Myco-TB kit combined with the MGIT™ system. For direct microscopy, the sensitivities of the Zephiran method and the Myco-TB method were equal (40%) when the centrifugation time was 20 min. The Myco-TB decontamination method is easy and rapid to perform. It is more sensitive for culture as compared to the Zephiran method and gives lower contamination levels when combined with the MGIT™ technique. When increasing the centrifugation step to 20 min, the sensitivity of direct microscopy is equal to the Zephiran method.
Myco-TB 程序（由 Copan（意大利布雷西亚）开发）。该技术与我们医院使用的泽菲兰去污方法进行了比较。2016 年 1 月至 2017 年 3 月期间，布鲁塞尔大学医院共纳入 387 份呼吸道标本（130 份气管/支气管抽吸物、172 份支气管肺泡灌洗物和 55 份痰液）。所有样本均分为两份：一份进行 Myco-TB 方法处理，另一份进行泽菲兰技术处理，然后进行培养。泽菲兰技术在固体培养基上的培养敏感性、Myco-TB 方法在固体培养基上的培养敏感性和 Myco-TB 方法与 MGIT 系统联合的培养敏感性分别为 67%、87%和 89%。两种固体培养基上的泽菲兰和 Myco-TB 方法的污染率分别为 22%和 4%，而 Myco-TB 试剂盒与 MGIT 系统联合的污染率仅为 4%。直接显微镜检查时，当离心时间为 20 分钟时，泽菲兰法和 Myco-TB 法的敏感性相等（40%）。Myco-TB 去污方法易于操作，快速。与泽菲兰方法相比，它对培养更敏感，与 MGIT 技术联合使用时污染水平更低。当增加离心步骤至 20 分钟时，直接显微镜检查的敏感性与泽菲兰方法相当。
