Smajdor Joanna, Piech Robert, Paczosa-Bator Beata
AGH University of Science and Technology, Faculty of Materials Science and Ceramics, 30-059 Kraków, al. Mickiewicza 30, Poland.
AGH University of Science and Technology, Faculty of Materials Science and Ceramics, 30-059 Kraków, al. Mickiewicza 30, Poland.
Steroids. 2018 Feb;130:1-6. doi: 10.1016/j.steroids.2017.12.007. Epub 2017 Dec 14.
In this study a new high sensitive method of spironolactone (SP) determination is proposed using voltammetric technique. As the working electrode amalgam film silver based electrode (Hg(Ag)FE) was applied, that resulted in obtaining low limit of spironolactone detection, ex. for the preconcentration time 45 s obtained detection limit was 4.7·10 mol L (1.96·10 g L). The linear response of spironolactone signal on mercury film electrode was obtained in the range of concentration from 15·10 to 3.0·10 mol L and the obtained repeatability measured as RSD was very good, with the value of 2.0% (n = 5; concentration 0.5·10 mol L). The proposed method was successfully applied and validated by studying the recovery in commercially available pharmaceutical formulations containing known amount of spironolactone.
在本研究中，提出了一种使用伏安技术测定螺内酯（SP）的新的高灵敏度方法。使用汞齐膜银基电极（Hg(Ag)FE）作为工作电极，这使得获得了较低的螺内酯检测限，例如，对于45秒的预浓缩时间，获得的检测限为4.7·10⁻⁸mol·L⁻¹（1.96·10⁻⁵g·L⁻¹）。在15·10⁻⁸至3.0·10⁻⁶mol·L⁻¹的浓度范围内获得了螺内酯信号在汞膜电极上的线性响应，并且获得的以相对标准偏差（RSD）衡量的重复性非常好，值为2.0%（n = 5；浓度为0.5·10⁻⁶mol·L⁻¹）。通过研究含有已知量螺内酯的市售药物制剂中的回收率，成功应用并验证了所提出的方法。
