Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Al. Mickiewicza 30, 30-059, Kraków, Poland.
Jagiellonian University Medical College, Department of Inorganic and Analytical Chemistry, Medyczna 9, 30-688, Kraków, Poland.
Talanta. 2022 Jan 15;237:122937. doi: 10.1016/j.talanta.2021.122937. Epub 2021 Oct 6.
New highly sensitive voltammetric method for captopril (CPT) determination was developed. The main novelty of the work was the application of a renewable amalgam film electrode (Hg(Ag)FE) for this purpose. During the research instrumental parameters of the developed method were optimized and were as follows: t = t = 5 ms, E = 5 mV, ΔE = 100 mV. Preconcentration potential and time were equal to 100 mV and 20 s, respectively. All measurements were conducted in electrolyte consisted of 0.1 M HClO. Limit of detection was calculated and was equal to 1.9 nM (0.39 ng mL) for 20 s preconcentration time and Hg(Ag)FE surface area approximately 11.2 mm. Linearity was achieved in the concentration range 0.05-1 μM. Repeatability of the method expressed as variation coefficient was estimated at 3.5% (0.15 μM CPT, n = 10). Applicability of the method was confirmed by analysis of tablets containing CPT and urine. Recoveries were in the range from 95 to 109% suggesting that the method might be assumed as accurate. Obtained results were also in good agreement with the producer declaration.
开发了一种新的用于卡托普利（CPT）测定的高灵敏度伏安法。这项工作的主要新颖之处在于为此目的应用了可再生汞齐膜电极（Hg(Ag)FE）。在研究中，优化了所开发方法的仪器参数，如下所示：t=t=5ms，E=5mV，ΔE=100mV。预浓缩电位和时间分别为 100mV 和 20s。所有测量均在由 0.1M HClO 组成的电解质中进行。检测限的计算结果为 1.9nM（20s 预浓缩时间和 Hg(Ag)FE 表面积约为 11.2mm 时为 0.39ngmL）。在线性范围内可实现 0.05-1μM 的浓度。方法的重复性以变异系数表示，估计值为 3.5%（0.15μM CPT，n=10）。通过分析含有 CPT 的片剂和尿液验证了该方法的适用性。回收率在 95%至 109%之间，表明该方法可以被认为是准确的。所得到的结果与生产商的声明也非常吻合。
