Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Ministry of Agriculture, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China.
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Ministry of Agriculture, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China.
J Dairy Sci. 2018 Mar;101(3):1915-1920. doi: 10.3168/jds.2017-13961. Epub 2017 Dec 14.
Lactobacillus casei Zhang is a probiotic strain originally isolated from koumiss. Previously, we showed that an alkaline shock protein (encoded by asp23) was involved in the adaptation of L. casei Zhang to gentamycin. In the present study, we compared the proteomes of the asp23 mutant and its parent strain grown in the presence of gentamycin. The results showed that 22 and 21 proteins were significantly up- and downregulated, respectively (>1.5-fold difference). By parallel reaction monitoring analysis, we further validated that specific membrane-associated proteins were important in regulating the antibiotics-induced cell wall stress. The findings provide insight into the physiological role of the asp23 gene in the growth response of L. casei when exposed to antibiotic stress.
干酪乳杆菌张是从马奶酒中分离出来的益生菌株。以前，我们发现碱性休克蛋白（由 asp23 编码）参与了干酪乳杆菌张对庆大霉素的适应。在本研究中，我们比较了 asp23 突变体及其亲株在庆大霉素存在下的蛋白质组。结果表明，分别有 22 个和 21 个蛋白分别显著上调和下调（差异>1.5 倍）。通过平行反应监测分析，我们进一步验证了特定的膜相关蛋白在调节抗生素诱导的细胞壁应激中的重要作用。这些发现为 asp23 基因在干酪乳杆菌暴露于抗生素应激时的生长反应中的生理作用提供了深入的了解。
