Basch P F, Nicolas C
Department of Health Research and Policy, Stanford University School of Medicine, California 94305.
Exp Parasitol. 1989 Feb;68(2):202-7. doi: 10.1016/0014-4894(89)90098-2.
To determine whether male worms provide any specific polypeptides to females, we produced extruded alginate fibers that resembled the size and shape of mature female worms. Males clasped these surrogate "female worms" and remained "paired" with them for long periods. On polyacrylamide gel electrophoresis, fibers clasped for several days showed bands at approximately 40 and 46 kDa which were never found in fibers incubated in the same medium but not clasped by males. We believe that these may be substances transferred from male worms during normal pairing. Males biosynthetically labeled with [14C]leucine were permitted to pair with fibers, which took up a broad range of polypeptides visualized on long autoradiographic exposure to gels.
为了确定雄虫是否会向雌虫提供任何特定的多肽，我们制作了挤压藻酸盐纤维，其大小和形状类似于成熟雌虫。雄虫会抓住这些替代的“雌虫”，并与它们长时间“配对”。在聚丙烯酰胺凝胶电泳中，被雄虫抓住数天的纤维在大约40 kDa和46 kDa处显示出条带，而在相同培养基中孵育但未被雄虫抓住的纤维中从未发现这些条带。我们认为这些可能是正常配对过程中雄虫转移的物质。用[14C]亮氨酸进行生物合成标记的雄虫与纤维配对，纤维摄取了在长时间凝胶放射自显影片上可见的多种多肽。
