Haseeb M A
Department of Microbiology and Immunology, State University of New York, Health Science Center, Brooklyn 11203, USA.
J Helminthol. 1998 Jun;72(2):123-6. doi: 10.1017/s0022149x00016291.
Incorporation of radiolabelled tyrosine was examined in males of Schistosoma mansoni maintained in vitro. Adult worms were recovered from mice and only those that were paired were used. Males and females were separated and placed in 4 degrees C RPMI 1640 containing 10% fetal calf serum (FCS). Individual males were incubated for 1 h either alone or with a female. Other males were incubated in a medium containing 40 ng ml-1 recombinant tumour necrosis factor alpha (rTNF alpha). Subsequently, males were incubated with or without females for 1 h in a medium containing 20 muCi ml-1 [14C]-tyrosine. All incubations were carried out in RPMI 1640 containing 10% FCS at 37 degrees C under 5% CO2. Untreated single males incorporated small amounts of labelled tyrosine. Pretreatment of single males with rTNF alpha did not affect their tyrosine incorporation (P = 0.693). However, males maintained in vitro with females incorporated 221% more tyrosine than the controls (P = 0.00008), suggesting that a chemical stimulus from the female affects tyrosine incorporation in males.
对体外培养的曼氏血吸虫雄虫的放射性标记酪氨酸掺入情况进行了检测。从小鼠体内获取成虫，仅使用配对的虫体。将雄虫和雌虫分离，置于含有10%胎牛血清（FCS）的4℃ RPMI 1640培养基中。将单个雄虫单独或与一条雌虫一起孵育1小时。其他雄虫在含有40 ng/ml重组肿瘤坏死因子α（rTNFα）的培养基中孵育。随后，将雄虫在含有20 μCi/ml [14C] -酪氨酸的培养基中与雌虫一起或不与雌虫一起孵育1小时。所有孵育均在含有10% FCS的RPMI 1640培养基中于37℃、5%二氧化碳条件下进行。未经处理的单个雄虫掺入少量标记酪氨酸。用rTNFα预处理单个雄虫对其酪氨酸掺入没有影响（P = 0.693）。然而，与雌虫一起体外培养的雄虫比对照组多掺入221%的酪氨酸（P = 0.00008），这表明来自雌虫的化学刺激会影响雄虫的酪氨酸掺入。
