借助高速机械DNA注射对小鼠培养细胞进行基因转化。
Genetic transformation of mouse cultured cells with the help of high-velocity mechanical DNA injection.
作者信息
Zelenin A V, Titomirov A V, Kolesnikov V A
机构信息
Engelhardt Institute of Molecular Biology, USSR Academy of Sciences, Moscow.
出版信息
FEBS Lett. 1989 Feb 13;244(1):65-7. doi: 10.1016/0014-5793(89)81163-9.
NIH 3T3 mouse cells were transfected by the plasmid pSV3neo (G418-resistant) with the help of high-velocity mechanical DNA injection based on the principle of bombarding cells with tungsten particles covered with the DNA. Stable transformants were obtained. Dot-hybridization and Southern analysis revealed the integration into the genome of 5-20 copies per cell of original plasmid DNA. The plasmid DNA was shown to have tandem organization.
基于用覆盖有DNA的钨颗粒轰击细胞的原理，借助高速机械DNA注射法，用质粒pSV3neo（对G418耐药）转染NIH 3T3小鼠细胞。获得了稳定的转化体。斑点杂交和Southern分析表明，每个细胞基因组中整合了5至20个拷贝的原始质粒DNA。质粒DNA显示具有串联结构。
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