在转移pPyLT1 DNA后建立的12种转基因小鼠品系中自主遗传元件的维持
Maintenance of autonomous genetic elements in twelve transgenic mouse strains established after transfer of pPyLT1 DNA.
作者信息
Léopold P, Vailly J, Cuzin F, Rassoulzadegan M
出版信息
Biochem Biophys Res Commun. 1986 Dec 30;141(3):1162-9. doi: 10.1016/s0006-291x(86)80166-8.
Maintenance and efficient meiotic segregation of autonomous genetic elements in four transgenic mouse strains established after micro-injection of plasmid pPyLT1 were previously reported. These findings are now extended to a total of twelve independent transgenic families. In spite of extensive rearrangements, half of these plasmids maintained the region of pBR322 necessary for shuttle transfer in E. coli. They all included mouse DNA sequences and the same, or closely related sequences were present in distinct mouse strains.
先前报道了在显微注射质粒pPyLT1后建立的四个转基因小鼠品系中自主遗传元件的维持和高效减数分裂分离。现在这些发现扩展到总共12个独立的转基因家族。尽管发生了广泛的重排，但这些质粒中有一半保留了在大肠杆菌中穿梭转移所需的pBR322区域。它们都包含小鼠DNA序列，并且在不同的小鼠品系中存在相同或密切相关的序列。
Zotero 插件