Shingaki Tomoya, Hiraguchi Yukiko, Gen Mari, Yoshino Shoko, Kumagai Yusuke, Ebishima Yuko, Owa Kenji, Otuji Kenta, Kondou Yasuto, Suehiro Yutaka
Department of Pediatrics, Center of Allergy and Clinical Immunology, Osaka Saiseikai Nakatsu Hospital.
Department of Pediatrics, Okinawa Kyodo Hospital.
Arerugi. 2017;66(10):1240-1243. doi: 10.15036/arerugi.66.1240.
We experienced a case of 10-year-old girl who developed hypersensitivity reactions after eating enokitake. The patient had food allergy to egg until 5 years old. When she was 4 years old, she ate enokitake with a hot-pot dish. Later, she felt itching in her mouth. Therefore, she never ate enokitake since that time. At the age of 10, she drank only the soup of enokitake with school lunch. After that she felt discomfort and itching in her oral cavity. The result of enokitake and other mushrooms (siitake, simeji, and eringi) skin prick to prick test were all positive. We performed Western blotting with enokitake extracts and the patient's serum. Enokitake protein's band (75kDa) reacted specifically with the patient's IgE. At the same time Western blotting was performed with the patient's serum of previously reported enokitake anaphylaxis, but a 75kDa band showing specific reaction in this case was not observed. This band we identified was a novel enokitake allergen.
我们遇到一例10岁女孩，食用金针菇后出现过敏反应。该患者5岁前对鸡蛋有食物过敏。4岁时，她吃了金针菇火锅。之后，她感到口腔瘙痒。因此，从那时起她就再也没吃过金针菇。10岁时，她在学校午餐时只喝了金针菇汤。之后她感到口腔不适和瘙痒。金针菇及其他蘑菇（香菇、真姬菇和杏鲍菇）的点刺试验结果均为阳性。我们用金针菇提取物和患者血清进行了蛋白质印迹分析。金针菇蛋白条带（75kDa）与患者的IgE发生特异性反应。同时，用先前报道的金针菇过敏患者的血清进行蛋白质印迹分析，但未观察到在该病例中显示特异性反应的75kDa条带。我们鉴定出的这条带是一种新的金针菇过敏原。
