Laboratory of Biochemistry, Graduate School of Integrated Pharmaceutical and Nutritional Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
TechnoSuruga Laboratory Co., Ltd., Shizuoka 424-0065, Japan.
Nutrients. 2023 Apr 29;15(9):2142. doi: 10.3390/nu15092142.
Several studies have reported the effects of the consumption of various mushroom species on the testes in animal experimental models. Mushrooms, including enokitake mushrooms (), and vegetables contain adenosine may affect testosterone production. Here, we aimed to elucidate the effects of enokitake and its active component, adenosine, on testosterone production in primary cultures of testicular cells in vivo using mice models and in vitro. The administration of enokitake ethanolic extract increased testosterone production in the cisplatin-impaired mouse model. The direct effect of mushroom extracts on testicular cells was examined and liquid chromatography-mass spectrometry analysis confirmed that the mushroom- and vegetable-induced increase in testosterone production mainly involved adenosine. Additionally, the administration of enokitake extract or adenosine to wet floor fatigue model mice promoted testicular testosterone production and enhanced Leydig cell function through insulin-like peptide three level upregulation. Structurally related compounds, including cordycepin, showed lower bioactivity than adenosine. This study showed that the ingestion of adenosine-containing mushrooms and vegetables may effectively increase testicular testosterone production. We conclude that mushrooms with a relatively high adenosine content, such as enokitake, may be useful against aging and fatigue.
已有几项研究报道了各种食用蘑菇品种对动物实验模型睾丸的影响。包括金针菇（）在内的蘑菇和蔬菜中含有的腺苷可能会影响睾丸激素的产生。在这里，我们旨在使用小鼠模型和体外实验阐明金针菇及其活性成分腺苷对睾丸细胞原代培养中睾丸激素产生的影响。金针菇醇提取物的给药增加了顺铂损伤小鼠模型中的睾丸激素产生。检查了蘑菇提取物对睾丸细胞的直接作用，液相色谱-质谱分析证实蘑菇和蔬菜诱导的睾丸激素产生增加主要涉及腺苷。此外，向湿地板疲劳模型小鼠给予金针菇提取物或腺苷可通过上调胰岛素样肽三级来促进睾丸睾酮的产生并增强黄体细胞功能。结构相关的化合物，包括虫草素，其生物活性低于腺苷。这项研究表明，摄入含腺苷的蘑菇和蔬菜可能有效地增加睾丸睾丸激素的产生。我们得出结论，金针菇等腺苷含量相对较高的蘑菇可能对衰老和疲劳有用。
