Cao Zhiyun, Zheng Liangpu, Zhao Jinyan, Zhuang Qunchuan, Hong Zhenfeng, Lin Wei
Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
Oncol Lett. 2017 Dec;14(6):7565-7570. doi: 10.3892/ol.2017.7075. Epub 2017 Sep 27.
The present study aimed to detect the impact of the ethanol extract of the seed (EELC) on angiogenesis in human umbilical vein endothelial cells (HUVECs). A chorioallantoic membrane (CAM) assay was used to detect the anti-angiogenic activity of EELC . , the effect of EELC on the proliferation, migration and angiogenesis of HUVECs was determined by an MTT assay, a wound healing assay and a tube formation assay, respectively. The vascular endothelial growth factor (VEGF)-A and VEGF receptor (VEGFR)-2 protein and mRNA level were measured with ELISA and reverse transcription-semi-quantitative polymerase chain reaction. It was observed that EELC significantly decreased the formation of new vessels in the CAM assay. EELC inhibited the proliferation and migration of HUVECs. The extent of tube formation by HUVECs was also reduced by EELC. In addition, EELC treatment reduced the level of VEGF-A and VEGFR-2 mRNA and protein. The results suggest that EELC inhibits tumor angiogenesis through inhibiting the proliferation and migration of HUVECs, and by downregulating VEGF and VEGFR.
本研究旨在检测莲子乙醇提取物（EELC）对人脐静脉内皮细胞（HUVECs）血管生成的影响。采用鸡胚绒毛尿囊膜（CAM）试验检测EELC的抗血管生成活性。分别通过MTT试验、伤口愈合试验和管腔形成试验测定EELC对HUVECs增殖、迁移和血管生成的影响。用酶联免疫吸附测定（ELISA）和逆转录-半定量聚合酶链反应检测血管内皮生长因子（VEGF）-A和VEGF受体（VEGFR）-2的蛋白和mRNA水平。观察到在CAM试验中EELC显著减少了新血管的形成。EELC抑制了HUVECs的增殖和迁移。EELC还降低了HUVECs的管腔形成程度。此外，EELC处理降低了VEGF-A和VEGFR-2的mRNA和蛋白水平。结果表明，EELC通过抑制HUVECs的增殖和迁移以及下调VEGF和VEGFR来抑制肿瘤血管生成。
