Hus Iwona, Bojarska-Junak Agnieszka, Kamińska Marzena, Dobrzyńska-Rutkowska Aneta, Szatan Karolina, Szymczyk Agnieszka, Kukiełka-Budny Bożena, Szczepanek Dariusz, Roliński Jacek
Department of Clinical Transplantology, Medical University of Lublin, 20-081 Lublin, Poland.
Department of Clinical Immunology, Medical University of Lublin, 20-093 Lublin, Poland.
Oncol Lett. 2017 Dec;14(6):7957-7964. doi: 10.3892/ol.2017.7232. Epub 2017 Oct 20.
T cells are important in B-cell non-Hodgkin's lymphoma immunity, however the function of T cell subsets, including natural killer (iNKT), T helper (Th)17, and T regulatory cells remains to be elucidated. The present study analyzed the frequencies of iNKT, Th17 and T regulatory cells in the peripheral blood of 41 patients with B-cell non-Hodgkin lymphoma at diagnosis, then during and following immunochemotherapy R-CHOP/R-CVP. At lymphoma diagnosis, iNKT and Th17 frequencies were decreased and T regulatory cell frequencies were increased compared with healthy control group. The Th17 cell percentage was lower in patients with a worse prognosis and at a more advanced clinical stage and in contrast, the percentage of T regulatory cells was increased in patients at advanced stages of lymphoma, compared to earlier stages. There was an increase of iNKT and Th17 cells following R-CHOP/R-CVP therapy. In patients that responded, both prior to and following-treatment, percentages of iNKT and Th17 were higher and T regulatory cells were lower compared with patients with subsequent disease progression. Taken together, the results obtained demonstrated the opposing effects of T cell subsets in B-cell lymphoma immunity, with iNKT and Th17 inhibiting and T regulatory cells enhancing tumor growth. These alterations may be caused by malignant B-cells, however there may also be an axis of inverse feedback between T regulatory cells and their interaction with Th17 and iNKT cells.
T细胞在B细胞非霍奇金淋巴瘤免疫中起重要作用，然而包括自然杀伤性T细胞（iNKT）、辅助性T细胞（Th）17和调节性T细胞在内的T细胞亚群的功能仍有待阐明。本研究分析了41例B细胞非霍奇金淋巴瘤患者在诊断时、免疫化疗R-CHOP/R-CVP期间及之后外周血中iNKT、Th17和调节性T细胞的频率。在淋巴瘤诊断时，与健康对照组相比，iNKT和Th17频率降低，调节性T细胞频率升高。预后较差、临床分期较晚的患者中Th17细胞百分比更低，相反，与早期相比，淋巴瘤晚期患者中调节性T细胞百分比升高。R-CHOP/R-CVP治疗后iNKT和Th17细胞增加。在有反应的患者中，治疗前和治疗后，与随后疾病进展的患者相比，iNKT和Th17的百分比更高，调节性T细胞更低。综上所述，所获得的结果表明T细胞亚群在B细胞淋巴瘤免疫中具有相反的作用，iNKT和Th17抑制肿瘤生长，调节性T细胞促进肿瘤生长。这些改变可能由恶性B细胞引起，然而调节性T细胞与其与Th17和iNKT细胞的相互作用之间也可能存在负反馈轴。
