State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei, China.
Medical Research Institute, Wuhan University, Wuhan, Hubei, China.
Proteomics. 2018 Jan;18(2). doi: 10.1002/pmic.201700403.
TBK1, STING, and MDA5 are important players within the antiviral innate immune response network. We mapped the interactome of endogenous TBK1, STING, and MDA5 by affinity enrichment MS in virally infected or uninfected THP-1 cells. Based on quantitative data of more than 2000 proteins and stringent statistical analysis, 58 proteins were identified as high-confidence interactors for at least one of three bait proteins. Our data indicated that TBK1 and MDA5 mostly interacted within preexisting protein networks, while STING interacted with different proteins with different viral infections. Functional analysis was performed on 17 interactors, and six were found to have functions in innate immune responses. We identified TTC4 as a TBK1 interactor and positive regulator of sendai virus-induced innate immunity.
TBK1、STING 和 MDA5 是抗病毒先天免疫反应网络中的重要参与者。我们通过亲和富集 MS 在病毒感染或未感染的 THP-1 细胞中绘制了内源性 TBK1、STING 和 MDA5 的互作组。基于超过 2000 种蛋白质的定量数据和严格的统计分析，有 58 种蛋白质被鉴定为至少一种诱饵蛋白的高可信度相互作用物。我们的数据表明，TBK1 和 MDA5 主要在预先存在的蛋白质网络中相互作用，而 STING 在不同的病毒感染中与不同的蛋白质相互作用。对 17 个相互作用物进行了功能分析，其中 6 个具有先天免疫反应的功能。我们鉴定出 TTC4 是 TBK1 的相互作用物和仙台病毒诱导的先天免疫的正调节剂。
