Moreno-Camacho José L, Calva-Espinosa Diana Y, Leal-Leyva Yoseli Y, Elizalde-Olivas Dolores C, Campos-Romero Abraham, Alcántar-Fernández Jonathan
Clinical Laboratory Division, Salud Digna para todos I.A.P. Culiacan, Sinaloa, México.
Clinical Microbiology Laboratory, National Reference Center, Salud Digna para todos I.A.P., Culiacan, Sinaloa, México.
Lab Med. 2017 Dec 22;49(1):e1-e8. doi: 10.1093/labmed/lmx079.
To validate the performance, reproducibility, and reliability of BD automated instruments in order to establish a fully automated clinical microbiology laboratory.
We used control strains and clinical samples to assess the accuracy, reproducibility, and reliability of the BD Kiestra WCA, the BD Phoenix, and BD Bruker MALDI-Biotyper instruments and compared them to previously established conventional methods. The following processes were evaluated: sample inoculation and spreading, colony counts, sorting of cultures, antibiotic susceptibility test, and microbial identification.
The BD Kiestra recovered single colonies in less time than conventional methods (e.g. E. coli, 7h vs 10h, respectively) and agreement between both methodologies was excellent for colony counts (κ=0.824) and sorting cultures (κ=0.821). Antibiotic susceptibility tests performed with BD Phoenix and disk diffusion demonstrated 96.3% agreement with both methods. Finally, we compared microbial identification in BD Phoenix and Bruker MALDI-Biotyper and observed perfect agreement (κ=1) and identification at a species level for control strains. Together these instruments allow us to process clinical urine samples in 36h (effective time).
The BD automated technologies have improved performance compared with conventional methods, and are suitable for its implementation in very busy microbiology laboratories.
为验证BD自动化仪器的性能、可重复性和可靠性，以建立一个全自动临床微生物实验室。
我们使用对照菌株和临床样本评估BD Kiestra WCA、BD Phoenix和BD Bruker MALDI-Biotyper仪器的准确性、可重复性和可靠性，并将它们与先前建立的传统方法进行比较。评估了以下过程：样本接种与铺展、菌落计数、培养物分类、抗生素敏感性试验和微生物鉴定。
BD Kiestra比传统方法更快地获得单菌落（例如，大肠杆菌分别为7小时和10小时），两种方法在菌落计数（κ=0.824）和培养物分类（κ=0.821）方面的一致性非常好。用BD Phoenix和纸片扩散法进行的抗生素敏感性试验显示两种方法的一致性为96.3%。最后，我们比较了BD Phoenix和Bruker MALDI-Biotyper的微生物鉴定结果，观察到完全一致（κ=1），并且对照菌株在种水平上得到了鉴定。这些仪器共同使我们能够在36小时（有效时间）内处理临床尿液样本。
与传统方法相比，BD自动化技术性能有所提高，适用于非常繁忙的微生物实验室。
