Bednarczyk M, Muc-Wierzgoń M, Waniczek D, Fatyga E, Klakla K, Mazurek U, Wierzgoń J
School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Department of Molecular Biology, Sosnowiec, Poland.
School of Public Health, Medical University of Silesia, Department of Internal Medicine, Bytom, Poland.
J Biol Regul Homeost Agents. 2017 Oct-Dec;31(4):923-927.
There is evidence that autophagy can play a dual role in tumor cells as a tumor suppressor, and a process involved in tumor cell survival. The aim of this work was to assess the expression of the genes engaged in the autophagy process in biopsies taken from the colon, confirmed as adenocarcinoma, and normal tissue and to relate them to the clinical stage of the tumor. A total of 20 pairs of surgically removed tumors and healthy (marginal) tissue samples from colorectal cancer patients at clinical stages (CS) I-IV were analyzed. Gene expression profile analysis was performed using HG-U133A microarrays. Differentially expressed genes were identified, using the PL-Grid Infrastructure. Only for CSI, there were two specific genes: FOXO1 and BNIP1; further in CSII LAMP2, MET and BCL2L, in CSIII HIF1A and 2 ID mRNAs for HGF and 18 genes were specific for CSIV in comparison to controls. PINK1 is the only gene that differentiates all transcriptome groups from controls. Furthermore, examination of the expression of genes associated with the autophagy process may allow for better knowledge and understanding of the processes occurring during the development of colon cancer. The presented genes may be used as prognostic markers of clinical stages of colorectal cancer, contributing to the development of new lines of therapy focused on reducing metastasis of the primary tumor.
有证据表明自噬在肿瘤细胞中可发挥双重作用——作为一种肿瘤抑制因子以及参与肿瘤细胞存活的一个过程。这项工作的目的是评估取自确诊为腺癌的结肠活检组织以及正常组织中参与自噬过程的基因的表达情况，并将它们与肿瘤的临床分期相关联。总共分析了20对取自临床分期（CS）I-IV期的结直肠癌患者的手术切除肿瘤和健康（边缘）组织样本。使用HG-U133A微阵列进行基因表达谱分析。利用PL-Grid基础设施识别差异表达基因。仅对于CS I期，有两个特定基因：FOXO1和BNIP1；在CS II期还有LAMP2、MET和BCL2L，在CS III期有HIF1A以及HGF的2个ID mRNA，与对照组相比，有18个基因在CS IV期是特异性的。PINK1是唯一能将所有转录组与对照组区分开来的基因。此外，对与自噬过程相关基因表达的检测可能有助于更好地了解和认识结肠癌发生发展过程中所发生的过程。所呈现的这些基因可用作结直肠癌临床分期的预后标志物，有助于开发专注于减少原发性肿瘤转移的新治疗方法。
