结直肠癌中、和基因的表达模式可能受微小RNA和CpG岛调控：一项计算机模拟研究
The Expression Patterns of , , and Genes in Colorectal Cancer Are Potentially Regulated by Micrornas and CpG Islands: An In Silico Study.
作者信息
Bednarczyk Martyna, Fatyga Edyta, Dzięgielewska-Gęsiak Sylwia, Waniczek Dariusz, Grabarek Beniamin, Zmarzły Nikola, Janikowska Grażyna, Muc-Wierzgoń Małgorzata
机构信息
Department of Internal Medicine, Medical University of Silesia in Katowice, 40-055 Katowice, Poland.
Department of Propedeutics Surgery, Chair of General, Colorectal and Polytrauma Surgery, Medical University of Silesia in Katowice, SHS in Katowice, 40-055 Katowice, Poland.
出版信息
J Clin Med. 2020 Dec 12;9(12):4020. doi: 10.3390/jcm9124020.
BACKGROUND
Autophagy plays a dual role of tumor suppression and tumor promotion in colorectal cancer. The study aimed to find those microRNAs (miRNAs) important in , , and regulation and to determine the possible role of the epigenetic changes in examined colorectal cancer using an in silico approach.
METHODS
A total of 44 pairs of surgically removed tumors at clinical stages I‒IV and healthy samples (marginal tissues) from patients' guts were analyzed. Analysis of the obtained results was conducted using the PL-Grid Infrastructure and Statistica 12.0 program. The miRNAs and CpG islands were estimated using the microrna.org database and MethPrimer program.
RESULTS
The autophagy-related genes were shown to be able to be regulated by miRNAs (-49 mRNA, -62 mRNA, -6 mRNA). It was observed that promotion regions containing at least one CpG region were present in the sequence of each gene.
CONCLUSIONS
The in silico analysis performed allowed us to determine the possible role of epigenetic mechanisms of regulation gene expression, which may be an interesting therapeutic target in the treatment of colorectal cancer.
背景
自噬在结直肠癌中发挥着肿瘤抑制和肿瘤促进的双重作用。本研究旨在寻找在[此处原文缺失具体内容]调控中重要的那些微小RNA（miRNA），并使用计算机模拟方法确定表观遗传变化在检测的结直肠癌中的可能作用。
方法
共分析了44对临床I-IV期手术切除的肿瘤以及来自患者肠道的健康样本（边缘组织）。使用PL-Grid基础设施和Statistica 12.0程序对所得结果进行分析。使用microrna.org数据库和MethPrimer程序估计miRNA和CpG岛。
结果
自噬相关基因显示能够受miRNA（-49 mRNA、-62 mRNA、-6 mRNA）调控。观察到每个基因序列中存在包含至少一个CpG区域的促进区域。
结论
所进行的计算机模拟分析使我们能够确定基因表达调控表观遗传机制的可能作用，这可能是结直肠癌治疗中一个有趣的治疗靶点。
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