Food Safety and Technology Research Centre, State Key Laboratory of Chirosciences and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region, China; State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) and Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen Research Institute of The Hong Kong Polytechnic University, Shenzhen, China.
State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation) and Shenzhen Key Laboratory of Food Biological Safety Control, Shenzhen Research Institute of The Hong Kong Polytechnic University, Shenzhen, China; University Research Facility in Chemical and Environmental Analysis, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong Special Administrative Region, China.
Anal Chim Acta. 2018 Jan 25;999:99-106. doi: 10.1016/j.aca.2017.11.006. Epub 2017 Nov 9.
In this study, direct ionization mass spectrometry (DI-MS) has been developed for rapid differentiation of Ganoderma (known as Lingzhi in Chinese), a very popular and valuable herbal medicine. Characteristic mass spectra can be generated by DI-MS directly from the raw herbal medicines with the application of a high voltage and solvents. Rapid differentiation of the Ganoderma species that are officially stated in the Chinese pharmacopoeia from easily confused Ganoderma species could be achieved based on this method, as the acquired DI-MS spectra showed that ganoderic acids, the major active components of Ganoderma, could be found only in the official Ganoderma species but not in the confused Ganoderma species. In addition, classification of wild and cultivated Ganoderma and potential differentiation of Ganoderma from different geographical locations could be accomplished based on principal component analysis (PCA) or hierarchical clustering analysis (HCA). The method is rapid, simple and reproducible, and can be further extended to analysis of other herbal medicines.
在这项研究中，直接电离质谱（DI-MS）已被开发用于快速区分灵芝（中文名为“灵芝”），灵芝是一种非常受欢迎且有价值的草药。通过 DI-MS，直接从原始草药中应用高电压和溶剂，即可生成特征质谱。基于该方法，可以快速区分中国药典中规定的灵芝物种与易混淆的灵芝物种，因为获得的 DI-MS 图谱表明，灵芝酸是灵芝的主要活性成分，只能在官方灵芝物种中找到，而在易混淆的灵芝物种中则找不到。此外，基于主成分分析（PCA）或层次聚类分析（HCA），可以完成野生和栽培灵芝的分类以及潜在的灵芝与不同地理位置的灵芝的区分。该方法快速、简单、重现性好，可进一步扩展到其他草药的分析。
