骨髓间充质干细胞改善去卵巢大鼠腮腺损伤。

Bone marrow-derived mesenchymal stem cells ameliorate parotid injury in ovariectomized rats.

机构信息

Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt.

出版信息

Cytotherapy. 2018 Feb;20(2):204-217. doi: 10.1016/j.jcyt.2017.10.003. Epub 2017 Dec 15.

DOI:10.1016/j.jcyt.2017.10.003

PMID:29254763

Abstract

BACKGROUND AIMS

Parotid hypofunction causes life-disrupting effects, and there are no effective medications for xerostomia. We hypothesized that mesenchymal stem cells (MSCs) have repairing effects on parotid glands of ovariectomized (OVX) rats.

METHODS

Forty-five adult female rats were divided into three equal groups: group I (Control group), group II (OVX-group) and group III (OVX rats that received MSCs at 4 and 8 weeks post-ovariectomy). At 12 weeks post-ovariectomy, histological (Masson's trichrome and periodic acid-Schiff with alcian blue stains), immunohistochemical (caspase-3 and CD44) and morphometric studies and salivary flow rate and saliva pH determination were carried out.

RESULTS

Histologically, the OVX group displayed numerous irregular vacuolated acini, thickened septa with marked cellular infiltration and vascular congestion. Degenerated organelles and few or irregular secretory granules with a different density were observed. Caspase-3-positive cells were highly expressed. MSC-treated glands exhibited a considerable degree of preservation of glandular architecture with numerous CD44-expressing and few caspase-3-expressing cells. Significant decrease of the salivary flow rate in the OVX group was detected, which reverted to normal levels in group III.

CONCLUSIONS

MSCs ameliorated the damaging effects of ovariectomy on the parotid glands.

摘要

背景目的

腮腺功能低下会导致生活质量受到影响，但目前尚无有效的口干症治疗药物。我们假设间充质干细胞（MSCs）对去卵巢（OVX）大鼠的腮腺具有修复作用。

方法

将 45 只成年雌性大鼠随机分为三组：I 组（对照组）、II 组（OVX 组）和 III 组（OVX 大鼠在去卵巢后 4 周和 8 周时接受 MSCs 治疗）。在去卵巢后 12 周，进行组织学（Masson 三色和过碘酸希夫-阿尔辛蓝染色）、免疫组织化学（半胱氨酸天冬氨酸蛋白酶-3 和 CD44）和形态计量学研究，并测定唾液流量和唾液 pH 值。

结果

组织学上，OVX 组显示出大量不规则空泡化的腺泡、增厚的隔室伴有明显的细胞浸润和血管充血。观察到退化的细胞器和稀疏或不规则的、密度不同的分泌颗粒。Caspase-3 阳性细胞高表达。MSC 治疗的腺体具有相当程度的腺体结构保存，CD44 表达阳性细胞较多，Caspase-3 表达阳性细胞较少。在 OVX 组中检测到唾液流量显著减少，而在 III 组中恢复到正常水平。