Mostafavinia Ataroalsadat, Dehdehi Leila, Ghoreishi Seyed Kamran, Hajihossainlou Behnam, Bayat Mohammad
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Statistics, University of Qom, Qom, Iran.
J Photochem Photobiol B. 2017 Oct;175:29-36. doi: 10.1016/j.jphotobiol.2017.08.021. Epub 2017 Aug 18.
Postmenopausal osteoporosis (PMOP) is considered by decreased bone strength that escalates the threat of fractures. Positive effects of photobiomodulation (PBM) with pulse wave have been demonstrated in cell culture and animal models. The aim of this study was to assess the in vivo effects of PBM on viability and calcium ion release of ovariectomy induced osteoporosis (OVX) - bone marrow derived mesenchymal stem cells (BMMSCs).
18 female rats were distributed into the following groups: 1) control healthy, 2) LASER-healthy (890nm, 80Hz, 1.5J/cm, three days weekly, 60days), 3) control OVX, 4) LASER-OVX, 5) Alendronate (Alen.)-OVX [0.5mg/kg, 5days per week, 60days], and 6) Alen.+LASER-OVX. Ovariectomy was done on rats of groups 3, 4, 5 and 6. After that all rats were euthanized and their MSC harvested and cultured in complete osteogenic medium. In all groups, BMMSC viability, and calcium colorimetric assay were performed.
We observed a significant increase in optical density (OD) of BMMSCs viability in LASER healthy group compared to control-OVX, Alen.-OVX, LASER-OVX, LASER+Alen.-OVX, groups. LASER+Alen.-OVX group displayed a significant escalation in OD of BMMSCs viability compared to LASER-OVX, Alen.-OVX, and control-OVX groups. There were a significant increase in calcium ion release of LASER-healthy group compared to control healthy, control-OVX, Alen.-OVX, LASER-OVX, and LASER+Alen.-OVX groups. LASER+Alen.-OVX group displayed a significant escalation in calcium ion release compared to LASER-OVX, Alen.-OVX, and control-OVX groups.
Pulse wave (PW) PBM significantly stimulated viability and cell proliferation of healthy BMMSCs compared to those of control-OVX, OVX-alendronate, OVX-LASER, and LASER+alendronate-OVX. In addition stimulatory effect of LASER+alendronate on viability and cell proliferation of OVX-BMMSCs compared to those of control-OVX, alendronate-OVX, and LASER-OVX groups were found.
绝经后骨质疏松症（PMOP）被认为是由于骨强度降低，增加了骨折风险。脉冲波光生物调节（PBM）在细胞培养和动物模型中已显示出积极作用。本研究旨在评估PBM对去卵巢诱导的骨质疏松症（OVX）-骨髓间充质干细胞（BMMSCs）活力和钙离子释放的体内影响。
将18只雌性大鼠分为以下几组：1）健康对照组；2）激光健康组（890nm，80Hz，1.5J/cm，每周3天，共60天）；3）OVX对照组；4）激光-OVX组；5）阿仑膦酸钠（Alen.）-OVX组[0.5mg/kg，每周5天，共60天]；6）阿仑膦酸钠+激光-OVX组。对第3、4、5和6组大鼠进行去卵巢手术。之后，将所有大鼠安乐死，收集其MSC并在完全成骨培养基中培养。对所有组进行BMMSC活力和钙比色测定。
与OVX对照组、阿仑膦酸钠-OVX组、激光-OVX组、激光+阿仑膦酸钠-OVX组相比，我们观察到激光健康组BMMSCs活力的光密度（OD）显著增加。与激光-OVX组、阿仑膦酸钠-OVX组和OVX对照组相比，激光+阿仑膦酸钠-OVX组BMMSCs活力的OD显著升高。与健康对照组、OVX对照组、阿仑膦酸钠-OVX组、激光-OVX组和激光+阿仑膦酸钠-OVX组相比，激光健康组的钙离子释放显著增加。与激光-OVX组、阿仑膦酸钠-OVX组和OVX对照组相比，激光+阿仑膦酸钠-OVX组的钙离子释放显著升高。
与OVX对照组、OVX-阿仑膦酸钠组、OVX-激光组和激光+阿仑膦酸钠-OVX组相比，脉冲波（PW）PBM显著刺激了健康BMMSCs的活力和细胞增殖。此外，与OVX对照组、阿仑膦酸钠-OVX组和激光-OVX组相比，发现激光+阿仑膦酸钠对OVX-BMMSCs的活力和细胞增殖有刺激作用。
