Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing, China.
Department of Pathology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing, China.
Pathol Res Pract. 2018 Feb;214(2):228-232. doi: 10.1016/j.prp.2017.11.013. Epub 2017 Nov 20.
It was found that G-protein-coupled receptor kinase 3 (GRK3) played key biological roles in some cancers. However, its associations with clinicopathologic features and prognosis in pancreatic ductal adenocarcinoma (PDAC) remain unknown.
Expression of GRK3 was detected, using tissue microarray-based immunohistochemistry, in paired formalin-fixed paraffin-embedded tumor and non-tumor samples from 165 patients with PDAC after curative resection, and was further correlated with clinicopathologic parameters and cancer-specific survival (CSS).
It was shown that GRK3 expression was much lower in tumor than in non-tumor tissues. Moreover, expression of GRK3 in tumor tissues was significantly associated with gender and T stage. Univariately, high GRK3 expression was predictive for favorable CSS, along with some conventional clinicopathologic variables. In multivariate Cox regression test, GRK3 expression remained to be a significant prognostic marker for PDAC. Finally, combination of GRK3 with some clinicopathologic variables, especially N stage, obtained more precise prediction for CSS.
Our data suggested that expression of GRK3 was down-regulated in PDAC and was an independent prognostic factor.
研究发现 G 蛋白偶联受体激酶 3（GRK3）在某些癌症中发挥着关键的生物学作用。然而，其与胰腺导管腺癌（PDAC）的临床病理特征和预后的关系尚不清楚。
使用组织微阵列免疫组织化学法，检测 165 例经根治性切除的 PDAC 患者配对的福尔马林固定石蜡包埋肿瘤和非肿瘤组织中 GRK3 的表达，并进一步与临床病理参数和癌症特异性生存（CSS）相关联。
结果表明，GRK3 在肿瘤组织中的表达明显低于非肿瘤组织。此外，肿瘤组织中 GRK3 的表达与性别和 T 分期显著相关。单变量分析显示，高 GRK3 表达与 CSS 预后良好相关，同时与一些传统的临床病理变量相关。多变量 Cox 回归试验表明，GRK3 表达仍然是 PDAC 的一个显著预后标志物。最后，GRK3 表达与一些临床病理变量的结合，特别是 N 分期，对 CSS 的预测更为准确。
我们的数据表明，GRK3 在 PDAC 中表达下调，是一个独立的预后因素。
