Long Mei, Yu Hua, Chen Li, Wu Guoyan, Zhao Siyue, Deng Wenwen, Chen Shujuan, Zhou Kang, Liu Shuliang, He Li, Ao Xiaoling, Yan Yubao, Ma Menggen, Wang Hongning, Davis Margaret A, Jones Lisa, Li Bei, Zhang Anyun, Zou Likou
College of Resources, Sichuan Agricultural University, Chengdu, 611130 People's Republic of China.
Sichuan Entry-Exit Inspection and Quarantine Bureau, Chengdu, 610041 People's Republic of China.
Gut Pathog. 2017 Dec 14;9:74. doi: 10.1186/s13099-017-0223-8. eCollection 2017.
is recognized as a common bacterial cause of foodborne diarrheal illness worldwide, and animal or its food products have been the most common vehicles of the infections. This study aimed to investigate the distribution of in two commercial layer farms and to determine the genetic relatedness between these strains. The isolates were serotyped by slide agglutination using commercial antisera and analyzed for genetic relatedness using pulsed-field gel electrophoresis (PFGE).
The internal environment had the highest prevalence of (14/15, 93.3%), followed by external environment (60/96, 62.5%) and egg samples (23/84, 27.3%). The prevalence of in the environment was significantly higher than that in egg samples ( < 0.05). The occurrence of in the internal environment (93.3%) was relatively higher than in the external environment (55.6-77.2%). The 111 isolates were distributed among 15 PFGE types, and the PFGE results suggested that there existed cross-contamination between these strains not only from eggs, but also from the environments.
The findings indicated ongoing cross-contamination inside or outside of the layer farms, and that could also spread along the egg production line.
被公认为全球食源性腹泻疾病常见的细菌病因，动物及其食品一直是该菌感染最常见的传播媒介。本研究旨在调查两个商业化蛋鸡养殖场中该菌的分布情况，并确定这些菌株之间的遗传相关性。使用商用抗血清通过玻片凝集法对该菌分离株进行血清分型，并使用脉冲场凝胶电泳（PFGE）分析其遗传相关性。
内部环境中该菌的患病率最高（14/15，93.3%），其次是外部环境（60/96，62.5%）和鸡蛋样本（23/84，27.3%）。环境中该菌的患病率显著高于鸡蛋样本中的患病率（P<0.05）。内部环境中该菌的发生率（93.3%）相对高于外部环境（55.6 - 77.2%）。111株分离株分布在15种PFGE型别中，PFGE结果表明这些菌株之间不仅存在来自鸡蛋的交叉污染，也存在来自环境的交叉污染。
研究结果表明蛋鸡养殖场内外存在该菌的持续交叉污染，并且该菌也可能沿鸡蛋生产线传播。
