Kimura I, Gotoh Y, Ozawa E
Division of Cell Biology, National Center for Neurology and Psychiatry, Tokyo, Japan.
In Vitro Cell Dev Biol. 1989 Mar;25(3 Pt 1):236-42. doi: 10.1007/BF02628460.
A mitogenic factor which promotes quail myoblast proliferation has been purified some 10(5)-fold from chick embryo extract by a combination of cation-exchange chromatography and heparin-affinity chromatography. The factor is eluted from heparin-Sepharose with 2 M NaCl and is a single-chain polypeptide with an apparent molecular weight of 15,000 to 17,000. It is active at subnanogram level in triggering the proliferation and thereby delaying temporarily fusion of myoblasts. It also stimulates the proliferation of quail fibroblasts in a similar effective concentration range. For both myoblasts and fibroblasts the dose-response to the factor is quantitatively and qualitatively comparable with that of bovine pituitary fibroblast growth factor. These observations strongly suggest that the factor very probably corresponds to chicken fibroblast growth factor or to a closely related molecule(s) and that it is possibly involved in the regulation of myogenesis.
一种促进鹌鹑成肌细胞增殖的促有丝分裂因子，通过阳离子交换色谱法和肝素亲和色谱法相结合，已从鸡胚提取物中纯化了约10⁵倍。该因子用2M NaCl从肝素 - 琼脂糖凝胶上洗脱下来，是一种单链多肽，表观分子量为15,000至17,000。它在亚纳克水平就具有活性，可触发成肌细胞增殖，从而暂时延迟其融合。在相似的有效浓度范围内，它还能刺激鹌鹑成纤维细胞的增殖。对于成肌细胞和成纤维细胞而言，该因子的剂量反应在数量和质量上与牛垂体成纤维细胞生长因子相当。这些观察结果强烈表明，该因子很可能与鸡成纤维细胞生长因子或密切相关的分子相对应，并且可能参与了肌发生的调控。
