• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

诱导多能干细胞分泌液（iPSC-cm）处理博来霉素损伤的大鼠肺后间质巨噬细胞的基因网络分析。

Gene Network Analysis of Interstitial Macrophages After Treatment with Induced Pluripotent Stem Cells Secretome (iPSC-cm) in the Bleomycin Injured Rat Lung.

机构信息

Department of Pulmonary Medicine, University Hospital Bern, 3010, Bern, Switzerland.

Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.

出版信息

Stem Cell Rev Rep. 2018 Jun;14(3):412-424. doi: 10.1007/s12015-017-9790-9.

DOI:10.1007/s12015-017-9790-9
PMID:29256173
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5960485/
Abstract

Idiopathic pulmonary fibrosis (IPF) is a complex disease involving various cell types. Macrophages are essential in maintenance of physiological homeostasis, wound repair and fibrosis in the lung. Macrophages play a crucial role in repair and remodeling by altering their phenotype and secretory pattern in response to injury. The secretome of induced pluripotent stem cells (iPSC-cm) attenuates injury and fibrosis in bleomycin injured rat lungs. In the current study, we evaluate the effect of iPSC-cm on gene expression and phenotype of interstitial macrophage in bleomycin injured rat lungs in vivo. iPSC-cm was intratracheally instilled 7 days after bleomycin induced lung injury and assessed 7 days later and single cell isolation was performed. Macrophages were FACS sorted and microarray analysis was performed. We characterized changes in the rat lung interstitial macrophages using transcriptional profiling. iPSC-cm reduced the total collagen content of the lung and reduced different macrophage populations. Gene set enrichment analysis revealed involvement of three essential pathways (a) immune modulation, (b) branching morphogenesis and (c) canonical Wnt signaling. This study demonstrates that iPSC-cm reduces fibrosis in bleomycin injured rat lung by partially altering the macrophages and regulating their gene expression.

摘要

特发性肺纤维化（IPF）是一种涉及多种细胞类型的复杂疾病。巨噬细胞在维持肺的生理稳态、伤口修复和纤维化中起着至关重要的作用。巨噬细胞通过改变其表型和分泌模式来应对损伤，在修复和重塑中发挥着关键作用。诱导多能干细胞（iPSC-cm）的分泌组可减轻博来霉素损伤的大鼠肺中的损伤和纤维化。在本研究中，我们评估了 iPSC-cm 对博来霉素诱导的大鼠肺损伤后体内间质巨噬细胞基因表达和表型的影响。在博来霉素诱导的肺损伤后 7 天，通过气管内滴注 iPSC-cm，并在 7 天后进行评估并进行单细胞分离。通过 FACS 分选巨噬细胞并进行微阵列分析。我们使用转录谱特征来描述大鼠肺间质巨噬细胞的变化。iPSC-cm 降低了肺的总胶原蛋白含量，并减少了不同的巨噬细胞群体。基因集富集分析显示了三个重要途径（a）免疫调节、（b）分支形态发生和（c）经典 Wnt 信号通路的参与。这项研究表明，iPSC-cm 通过部分改变巨噬细胞并调节其基因表达来减少博来霉素损伤的大鼠肺中的纤维化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/8d4f56bd5e9f/12015_2017_9790_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/050e24bd266e/12015_2017_9790_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/92eed660dc11/12015_2017_9790_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/a73858fd6928/12015_2017_9790_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/073be94b6ecc/12015_2017_9790_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/6aaa03e34a69/12015_2017_9790_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/024ad6bb60cb/12015_2017_9790_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/3ed2035ee7f8/12015_2017_9790_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/e6328e3caca8/12015_2017_9790_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/715e9fdc1cda/12015_2017_9790_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/8d4f56bd5e9f/12015_2017_9790_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/050e24bd266e/12015_2017_9790_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/92eed660dc11/12015_2017_9790_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/a73858fd6928/12015_2017_9790_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/073be94b6ecc/12015_2017_9790_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/6aaa03e34a69/12015_2017_9790_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/024ad6bb60cb/12015_2017_9790_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/3ed2035ee7f8/12015_2017_9790_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/e6328e3caca8/12015_2017_9790_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/715e9fdc1cda/12015_2017_9790_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00ff/5960485/8d4f56bd5e9f/12015_2017_9790_Fig10_HTML.jpg

相似文献

1
Gene Network Analysis of Interstitial Macrophages After Treatment with Induced Pluripotent Stem Cells Secretome (iPSC-cm) in the Bleomycin Injured Rat Lung.诱导多能干细胞分泌液（iPSC-cm）处理博来霉素损伤的大鼠肺后间质巨噬细胞的基因网络分析。
Stem Cell Rev Rep. 2018 Jun;14(3):412-424. doi: 10.1007/s12015-017-9790-9.
2
The secretome of induced pluripotent stem cells reduces lung fibrosis in part by hepatocyte growth factor.诱导多能干细胞的分泌组部分通过肝细胞生长因子减轻肺纤维化。
Stem Cell Res Ther. 2014 Nov 10;5(6):123. doi: 10.1186/scrt513.
3
Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro.iPSC 分泌组的相互作用组分析及其对体外巨噬细胞的影响。
Int J Mol Sci. 2021 Jan 19;22(2):958. doi: 10.3390/ijms22020958.
4
Exosomes derived from induced pluripotent stem cells suppresses M2-type macrophages during pulmonary fibrosis via miR-302a-3p/TET1 axis.诱导多能干细胞衍生的外泌体通过 miR-302a-3p/TET1 轴在肺纤维化中抑制 M2 型巨噬细胞。
Int Immunopharmacol. 2021 Oct;99:108075. doi: 10.1016/j.intimp.2021.108075. Epub 2021 Aug 17.
5
Alpha-Klotho Enrichment in Induced Pluripotent Stem Cell Secretome Contributes to Antioxidative Protection in Acute Lung Injury.α-klotho 蛋白在诱导多能干细胞分泌组中的富集有助于急性肺损伤的抗氧化保护。
Stem Cells. 2018 Apr;36(4):616-625. doi: 10.1002/stem.2752. Epub 2017 Dec 25.
6
Induced pluripotent stem cells mediate the release of interferon gamma-induced protein 10 and alleviate bleomycin-induced lung inflammation and fibrosis.诱导多能干细胞介导干扰素γ诱导蛋白 10 的释放，减轻博来霉素诱导的肺炎症和纤维化。
Shock. 2013 Mar;39(3):261-70. doi: 10.1097/SHK.0b013e318285f2e2.
7
Induced pluripotent stem cell-derived lung alveolar epithelial type II cells reduce damage in bleomycin-induced lung fibrosis.诱导多能干细胞衍生的肺Ⅱ型肺泡上皮细胞减轻博来霉素诱导的肺纤维化损伤。
Stem Cell Res Ther. 2020 Jun 3;11(1):213. doi: 10.1186/s13287-020-01726-3.
8
Kinetics of macrophage subpopulations and expression of monocyte chemoattractant protein-1 (MCP-1) in bleomycin-induced lung injury of rats studied by a novel monoclonal antibody against rat MCP-1.利用抗大鼠单核细胞趋化蛋白-1（MCP-1）的新型单克隆抗体研究博来霉素诱导的大鼠肺损伤中巨噬细胞亚群的动力学及MCP-1的表达。
J Leukoc Biol. 1994 Dec;56(6):741-50. doi: 10.1002/jlb.56.6.741.
9
Flow cytometric analysis of the leukocyte landscape during bleomycin-induced lung injury and fibrosis in the rat.流式细胞术分析博来霉素诱导的大鼠肺损伤和纤维化过程中的白细胞特征。
Am J Physiol Lung Cell Mol Physiol. 2019 Jul 1;317(1):L109-L126. doi: 10.1152/ajplung.00176.2018. Epub 2019 May 1.
10
Induced pluripotent stem cell-conditioned medium suppresses pulmonary fibroblast-to-myofibroblast differentiation via the inhibition of TGF-β1/Smad pathway.诱导多能干细胞条件培养基通过抑制 TGF-β1/Smad 通路抑制肺成纤维细胞向肌成纤维细胞分化。
Int J Mol Med. 2018 Jan;41(1):473-484. doi: 10.3892/ijmm.2017.3199. Epub 2017 Oct 19.

引用本文的文献

1
Stem cell-based therapy for fibrotic diseases: mechanisms and pathways.基于干细胞的纤维化疾病治疗：机制与途径。
Stem Cell Res Ther. 2024 Jun 18;15(1):170. doi: 10.1186/s13287-024-03782-5.
2
Induced pluripotent stem cell-based therapies for organ fibrosis.基于诱导多能干细胞的器官纤维化治疗方法。
Front Bioeng Biotechnol. 2023 May 18;11:1119606. doi: 10.3389/fbioe.2023.1119606. eCollection 2023.
3
Anti-Fibrotic Effect of SDF-1β Overexpression in Bleomycin-Injured Rat Lung.SDF-1β过表达对博来霉素损伤大鼠肺的抗纤维化作用

本文引用的文献

1
Avoiding the pitfalls of gene set enrichment analysis with SetRank.使用SetRank避免基因集富集分析的陷阱。
BMC Bioinformatics. 2017 Mar 4;18(1):151. doi: 10.1186/s12859-017-1571-6.
2
Regulatory T Cells in Idiopathic Pulmonary Fibrosis: Too Much of a Good Thing?特发性肺纤维化中的调节性T细胞：好事过头了？
Am J Pathol. 2016 Aug;186(8):1978-1981. doi: 10.1016/j.ajpath.2016.06.002. Epub 2016 Jun 23.
3
The Relationship between Gene Network Structure and Expression Variation among Individuals and Species.基因网络结构与个体及物种间表达变异的关系。
Pharmaceutics. 2022 Aug 27;14(9):1803. doi: 10.3390/pharmaceutics14091803.
4
Lung Fibrosis and Fibrosis in the Lungs: Is It All about Myofibroblasts?肺纤维化与肺部的纤维化：一切都与肌成纤维细胞有关吗？
Biomedicines. 2022 Jun 15;10(6):1423. doi: 10.3390/biomedicines10061423.
5
Therapeutic stem cell-derived alveolar-like macrophages display bactericidal effects and resolve Pseudomonas aeruginosa-induced lung injury.治疗性干细胞衍生的肺泡样巨噬细胞具有杀菌作用，并能解决铜绿假单胞菌引起的肺损伤。
J Cell Mol Med. 2022 May;26(10):3046-3059. doi: 10.1111/jcmm.17324. Epub 2022 Apr 20.
6
Basal-Like Cell-Conditioned Medium Exerts Anti-Fibrotic Effects and .基底样细胞条件培养基发挥抗纤维化作用并且……（原文不完整）
Front Bioeng Biotechnol. 2022 Mar 8;10:844119. doi: 10.3389/fbioe.2022.844119. eCollection 2022.
7
Role of nuclear factor-kappa B in bleomycin induced pulmonary fibrosis and the probable alleviating role of ginsenoside: histological, immunohistochemical, and biochemical study.核因子-κB在博来霉素诱导的肺纤维化中的作用及人参皂苷可能的缓解作用：组织学、免疫组织化学和生化研究
Anat Cell Biol. 2021 Dec 31;54(4):448-464. doi: 10.5115/acb.21.068.
8
Tenascin-C: Friend or Foe in Lung Aging?肌腱蛋白-C：肺部衰老中的朋友还是敌人？
Front Physiol. 2021 Oct 27;12:749776. doi: 10.3389/fphys.2021.749776. eCollection 2021.
9
Interactome Analysis of iPSC Secretome and Its Effect on Macrophages In Vitro.iPSC 分泌组的相互作用组分析及其对体外巨噬细胞的影响。
Int J Mol Sci. 2021 Jan 19;22(2):958. doi: 10.3390/ijms22020958.
10
Inhalational delivery of induced pluripotent stem cell secretome improves postpneumonectomy lung structure and function.诱导多能干细胞 secretome 的吸入式递送改善了全肺切除术后的肺结构和功能。
J Appl Physiol (1985). 2020 Nov 1;129(5):1051-1061. doi: 10.1152/japplphysiol.00205.2020. Epub 2020 Sep 10.
PLoS Genet. 2015 Aug 28;11(8):e1005398. doi: 10.1371/journal.pgen.1005398. eCollection 2015 Aug.
4
Pulmonary macrophages: key players in the innate defence of the airways.肺巨噬细胞：气道先天防御的关键细胞。
Thorax. 2015 Dec;70(12):1189-96. doi: 10.1136/thoraxjnl-2015-207020. Epub 2015 Aug 18.
5
The significance of macrophage polarization subtypes for animal models of tissue fibrosis and human fibrotic diseases.巨噬细胞极化亚型在组织纤维化动物模型和人类纤维化疾病中的意义。
Clin Transl Med. 2015 Feb 7;4:2. doi: 10.1186/s40169-015-0047-4. eCollection 2015.
6
limma powers differential expression analyses for RNA-sequencing and microarray studies.limma为RNA测序和微阵列研究提供差异表达分析的动力。
Nucleic Acids Res. 2015 Apr 20;43(7):e47. doi: 10.1093/nar/gkv007. Epub 2015 Jan 20.
7
Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis.特发性肺纤维化急性加重期的巨噬细胞活化
PLoS One. 2015 Jan 15;10(1):e0116775. doi: 10.1371/journal.pone.0116775. eCollection 2015.
8
The secretome of induced pluripotent stem cells reduces lung fibrosis in part by hepatocyte growth factor.诱导多能干细胞的分泌组部分通过肝细胞生长因子减轻肺纤维化。
Stem Cell Res Ther. 2014 Nov 10;5(6):123. doi: 10.1186/scrt513.
9
STRING v10: protein-protein interaction networks, integrated over the tree of life.STRING v10：整合了整个生命之树的蛋白质-蛋白质相互作用网络。
Nucleic Acids Res. 2015 Jan;43(Database issue):D447-52. doi: 10.1093/nar/gku1003. Epub 2014 Oct 28.
10
Macrophage activation and polarization: nomenclature and experimental guidelines.巨噬细胞激活与极化：命名及实验指南
Immunity. 2014 Jul 17;41(1):14-20. doi: 10.1016/j.immuni.2014.06.008.