Elal Mus Tulay, Cetinkaya Figen, Cibik Recep, Soyutemiz Gul Ece, Simsek Husniye, Coplu Nilay
1 Department of Food Processing, Vocational School of Keles, University of Uludag , Bursa , Turkey.
2 Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, University of Uludag , Bursa , Turkey.
Acta Vet Hung. 2017 Dec;65(4):461-474. doi: 10.1556/004.2017.044.
In this study, the presence of genes responsible for the pathogenicity and antibiotic resistance profile of enterococci isolated from various foodstuffs of animal origin was investigated. The percentage prevalence of enterococci was 54.1% (203/375) and the average count was found to be 3.81 log cfu/ml-g. Species-specific primers revealed Enterococcus faecalis as the predominant species carrying one or more virulence-associated traits of efa, gelE, ace, esp and agg genetic markers. Only one E. faecium isolate (from milk) was positive for the esp gene. Regarding antibiotic resistance, the highest frequency of resistance was observed for tetracycline (21.7%), followed by quinupristin/dalfopristin (13.3%), ciprofloxacin (2.0%), penicillin (2.0%), linezolid (1.0%), ampicillin (1.0%), streptomycin (1.0%), and gentamicin (0.5%). Enterococcus faecalis showed a higher prevalence of antibiotic resistance than other enterococci. The percentage of multidrug resistance among the isolates was 3.4%. Twenty-nine E. faecalis isolates (26.6%) carrying one of the virulence-associated traits were at the same time resistant to at least one antibiotic. Our results show that foods of animal origin, including ready-to-eat products, may be reservoirs of antibiotic-resistant and potentially virulent enterococci.
在本研究中，对从各种动物源性食品中分离出的肠球菌的致病性和抗生素抗性谱相关基因的存在情况进行了调查。肠球菌的检出率为54.1%（203/375），平均菌数为3.81 log cfu/ml - g。种特异性引物显示粪肠球菌是携带efa、gelE、ace、esp和agg遗传标记中一种或多种与毒力相关特征的主要菌种。仅一株屎肠球菌分离株（来自牛奶）的esp基因呈阳性。关于抗生素抗性，观察到对四环素的抗性频率最高（21.7%），其次是奎奴普丁/达福普汀（13.3%）、环丙沙星（2.0%）、青霉素（2.0%）、利奈唑胺（1.0%）、氨苄西林（1.0%）、链霉素（1.0%）和庆大霉素（0.5%）。粪肠球菌的抗生素抗性患病率高于其他肠球菌。分离株中的多重耐药率为3.4%。29株携带一种与毒力相关特征的粪肠球菌分离株（26.6%）同时对至少一种抗生素耐药。我们的结果表明，包括即食产品在内的动物源性食品可能是耐抗生素和具有潜在毒性的肠球菌的储存库。
