Subramanian Subha, Braun Patricia R, Han Shizhong, Potash James B
Roy J. and Lucille A. Carver College of Medicine.
Department of Psychiatry.
Psychiatr Genet. 2018 Feb;28(1):12-15. doi: 10.1097/YPG.0000000000000189.
The objective of this study was to investigate the relationship between methylation patterns of the histone deacetylase 4 gene and eating disorders in a site previously associated with anorexia nervosa (AN). Women with AN (N=28) or bulimia nervosa (BN) (N=19) were age-matched and sex-matched to controls (N=45). We obtained saliva-derived DNA and use bisulfite pyrosequencing to examine region-specific methylation differences between cases and controls. The region assayed includes 15 CpGs. We found no significant association between the previously implicated CpG and either AN or BN. We found that three CpGs were nominally associated with AN (P=0.02-0.03); the largest difference was a 9% hypermethylation in AN. One CpG was nominally associated with BN (P=0.04), with 4% hypomethylation. None of these results remained significant after correction for multiple testing. We did not replicate previous findings, though through expanded coverage, we identified additional CpGs that were nominally associated with eating disorders.
本研究的目的是在一个先前与神经性厌食症（AN）相关的地点，调查组蛋白去乙酰化酶4基因的甲基化模式与饮食失调之间的关系。将患有AN（N = 28）或神经性贪食症（BN）（N = 19）的女性与年龄和性别相匹配的对照组（N = 45）进行比较。我们获取了唾液来源的DNA，并使用亚硫酸氢盐焦磷酸测序来检测病例组和对照组之间区域特异性的甲基化差异。检测的区域包括15个CpG。我们发现先前涉及的CpG与AN或BN之间均无显著关联。我们发现有三个CpG与AN存在名义上的关联（P = 0.02 - 0.03）；最大差异是AN中甲基化增加9%。有一个CpG与BN存在名义上的关联（P = 0.04），甲基化减少4%。经过多重检验校正后，这些结果均无统计学意义。我们没有重复先前的研究结果，不过通过扩大覆盖范围，我们发现了其他与饮食失调存在名义上关联的CpG。
