Shao Lin-Nan, Yang Yang, Zhang Shu-Ting, Zhou Shi-Hang, Duan Ying, Meng Qing-Li, Liang Xiao-Hua, Yu Wei-Jian
a Blood Group Research Department, Dalian Blood Center , Dalian , Liaoning , China.
b The Third Department of Urology (Renal Transplant) , Dalian Friendship Hospital , Dalian , Liaoning , China.
Immunol Invest. 2018 Feb;47(2):212-219. doi: 10.1080/08820139.2017.1416397. Epub 2017 Dec 19.
End-stage renal disease (ESRD), the last stage of chronic renal failure, is a global health problem. The number of ESRD patients worldwide is increasing faster than the number of kidneys available per year for renal transplantation. Most of the ESRD patients are awaiting renal transplantation. The immune response to the transplanted kidney is directed mainly against mismatched human leukocyte antigen (HLA) glycoproteins expressed on donor tissues. Thus, the analysis of HLA allele and haplotype polymorphisms is valuable not only for identifying ESRD susceptibility factors but also to improve graft survival.
In this study, 163 Han ESRD patients were recruited to participate. The blood samples were genotyped by sequence-specific oligonucleotide method. A group of 14,529 healthy Chinese Han individuals registered at the Dalian Blood Center as bone marrow donors, living in the same region and of the same ethnicity, were used as controls.
We found that only one allele, HLA-DRB112, showed a positive association with ESRD (p = 0.004, p = 0.028, odds ratio = 1.530, 95% confidence interval = 1.147-2.041); A02-B40-DRB109, A02-B40-DRB112, A24-B15-DRB112, and B40-DRB112 were significantly more frequent in ESRD patients after Bonferroni correction (p < 0.05).
They were potentially valuable predictors for evaluating the risk of ESRD in the Dalian Han population.
终末期肾病（ESRD）是慢性肾衰竭的最后阶段，是一个全球性的健康问题。全球ESRD患者数量的增长速度超过了每年可用于肾移植的肾脏数量。大多数ESRD患者正在等待肾移植。对移植肾的免疫反应主要针对供体组织上表达的不匹配人类白细胞抗原（HLA）糖蛋白。因此，分析HLA等位基因和单倍型多态性不仅对于确定ESRD易感性因素有价值，而且对于提高移植肾存活率也有价值。
在本研究中，招募了163名汉族ESRD患者参与。通过序列特异性寡核苷酸方法对血样进行基因分型。将一组在大连血液中心登记为骨髓供体、生活在同一地区且种族相同的14529名健康中国汉族个体作为对照。
我们发现只有一个等位基因HLA-DRB112与ESRD呈正相关（p = 0.004，p = 0.028，优势比 = 1.530，95%置信区间 = 1.147 - 2.041）；经Bonferroni校正后，A02-B40-DRB109、A02-B40-DRB112、A24-B15-DRB112和B40-DRB112在ESRD患者中的出现频率显著更高（p < 0.05）。
它们可能是评估大连汉族人群ESRD风险的潜在有价值的预测指标。
