Sina M, Dirandeh E, Deldar H, Shohreh B
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari, Mazandaran, Iran.
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari, Mazandaran, Iran.
Theriogenology. 2018 Mar 1;108:262-268. doi: 10.1016/j.theriogenology.2017.12.020. Epub 2017 Dec 14.
This study was undertaken to determine the effect of inflammatory status on different patterns of postpartum luteal activity and reproductive performance of Holstein cows during early lactation. The cows (n = 75) averaged 3.4 ± 1.2 (mean ± SEM) in parity and 3.1 ± 0.2 (mean ± SEM) in body condition score at calving. Transrectal ultrasonography was performed twice weekly from day 10-60 postpartum to consider ovarian dynamics. Plasma concentrations of progesterone (P4) and estradiol (E2) were measured twice weekly and plasma levels of tumor necrosis factor alpha (TNF-α) and lipopolysacchride binding protein (LBP) were measured weekly (week 3-6 postpartum). Based on plasma P4 and E2 results, 34 (45.3%) cows had normal luteal activity (NLA), whereas 19 (25.3%), 12 (16.0%), 7 (9.3%) and 3 (4.0%) cows had prolonged luteal phase (PLP), delayed first ovulation (DO), anovulation (AO) and short luteal phase (SLP), respectively. Plasma TNF-α and LBP concentrations were affected by postpartum luteal activity (NLA, SLP and PLP), ovulatory status (DO and AO) and number of weeks postpartum (P < .05). These concentrations were greater in cows with PLP (P < .05) as compared to NLA cows, and in cows that had delayed ovulation or anovulation compared to ovulated cows (P < .05). Cows with PLP had greater open days and lower conception rate as compared to NLA cows (P < .05). Healthy cows had a larger CL and greater plasma estradiol and progesterone concentrations at first and second cycle postpartum compared to inflamed cows and followed it with greater fertility (P < .05). In conclusion, inflammatory statuses were different in high-producing dairy cows showing PLP, AO and DO in comparison with the postpartum normal luteal activity (NLA) cows that influenced reproduction outcomes.
本研究旨在确定炎症状态对荷斯坦奶牛泌乳早期产后不同黄体活动模式及繁殖性能的影响。这些奶牛（n = 75）产次平均为3.4 ± 1.2（均值 ± 标准误），产犊时体况评分平均为3.1 ± 0.2（均值 ± 标准误）。产后第10天至60天每周进行两次经直肠超声检查以观察卵巢动态。每周两次测定血浆孕酮（P4）和雌二醇（E2）浓度，每周（产后第3至6周）测定血浆肿瘤坏死因子α（TNF-α）和脂多糖结合蛋白（LBP）水平。根据血浆P4和E2结果，34头（45.3%）奶牛黄体活动正常（NLA），而19头（25.3%）、12头（16.0%）、7头（9.3%）和3头（4.0%）奶牛分别出现黄体期延长（PLP）、首次排卵延迟（DO）、不排卵（AO）和黄体期缩短（SLP）。血浆TNF-α和LBP浓度受产后黄体活动（NLA、SLP和PLP）、排卵状态（DO和AO）及产后周数影响（P < 0.05）。与NLA奶牛相比，PLP奶牛这些浓度更高（P < 0.05），与已排卵奶牛相比，排卵延迟或不排卵奶牛这些浓度更高（P < 0.05）。与NLA奶牛相比，PLP奶牛空怀天数更多，受胎率更低（P < 0.05）。与炎症奶牛相比，健康奶牛产后第一和第二周期黄体更大，血浆雌二醇和孕酮浓度更高，且繁殖力更强（P < 0.05）。总之，与产后黄体活动正常（NLA）的奶牛相比，高产奶牛出现PLP、AO和DO时炎症状态不同，这影响了繁殖结果。
