Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari, Mazandaran, Iran.
Department of Animal Science, Sari Agricultural Sciences and Natural Resources University, P.O. Box 578, Sari, Mazandaran, Iran.
Theriogenology. 2019 Jul 15;133:97-103. doi: 10.1016/j.theriogenology.2019.04.034. Epub 2019 Apr 30.
The role of oxidative stress in the control of female reproduction has not been fully elucidated in ruminants; however, it seems that antioxidants can make influence to the reproductive axis at different levels. Therefore, the aim of this study was to determine the relationship between antioxidant status and concentrations of trace minerals (chromium (Cr), copper (Cu), iron (Fe), and zinc (Zn)) with postpartum luteal activity and fertility in Holstein dairy cows. The cows (n = 100, a parity range of 2-5, and a body condition score (BCS) of 3.0 ± 0.25, mean ± SEM) were assigned to the experiment at second week post calving. Ovarian follicular dynamics were monitored twice a week (from 3rd to 6th weeks post calving) by transrectal ultrasonography (US). Blood samples were collected twice weekly from the 3rd to the 6th weeks post calving at timed artificial insemination (TAI), and days 32 and 50 post AI to determine superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), and trace mineral concentrations. There were associations between plasma concentrations of SOD, GPX, and TAC with postpartum luteal activity (PLA, P = 0.01) and ovulation (P = 0.03). Mean plasma SOD and GPX activities and TAC levels (U/mL) were greater in cows with normal luteal activity (NLA) than prolonged luteal phase (PLP) and anovulation (AO) cows, as well as in ovulated compared to AO cows (P = 0.03). Pregnant cows had greater levels of SOD, GPX, and TAC (U/mL) at TAI than non-pregnant cows (P = 0.01). Plasma Cu and Zn concentrations increased in pregnant compared to non-pregnant cows at TAI. In conclusion, antioxidant levels and Cu and Zn concentrations were associated with PLA and fertility.
氧化应激在反刍动物繁殖控制中的作用尚未完全阐明；然而，抗氧化剂似乎可以在不同水平上影响生殖轴。因此，本研究旨在确定抗氧化状态与痕量矿物质（铬（Cr）、铜（Cu）、铁（Fe）和锌（Zn））浓度与荷斯坦奶牛产后黄体活性和生育力的关系。奶牛（n=100，产次范围为 2-5，体况评分（BCS）为 3.0±0.25，平均值±SEM）在产后第二周被分配到实验中。通过直肠超声（US）每周两次监测卵巢卵泡动态（产后第 3 至第 6 周）。产后第 3 至第 6 周每周两次从定时人工授精（TAI）采集血液样本，并在产后第 32 天和第 50 天采集血液样本，以确定超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPX）、总抗氧化能力（TAC）和痕量矿物质浓度。SOD、GPX 和 TAC 的血浆浓度与产后黄体活性（PLA，P=0.01）和排卵（P=0.03）之间存在关联。具有正常黄体活性（NLA）的奶牛的血浆 SOD 和 GPX 活性和 TAC 水平（U/mL）均高于黄体期延长（PLP）和无排卵（AO）奶牛，以及与 AO 奶牛相比排卵奶牛（P=0.03）。TAI 时，妊娠奶牛的 SOD、GPX 和 TAC（U/mL）水平高于非妊娠奶牛（P=0.01）。与非妊娠奶牛相比，TAI 时妊娠奶牛的血浆 Cu 和 Zn 浓度增加。总之，抗氧化剂水平以及 Cu 和 Zn 浓度与 PLA 和生育力有关。
