Department of BiologyAppalachian State University, Boone, North Carolina, USA.
Department of BiologyAppalachian State University, Boone, North Carolina, USA
J Mol Endocrinol. 2018 Jan;60(1):17-28. doi: 10.1530/JME-17-0179.
A timely and complete uterine cervical tissue repair postpartum is of necessity to prevent obstetrical complications, such as cervicitis, ectropion, hemorrhage, repeated miscarriages or abortions and possibly preterm labor and malignancies. We recently characterized the morphological alterations, as well as changes in angiogenic expression profile in a mice uterine cervix during the immediate postpartum period. Here, we build on this previous study using a proteomic analysis to profile postpartum tissue changes in mice cervix during the same period, the first 48 h of postpartum. The current proteomics data reveal a variable expression of several intermediate filaments, cytoskeletal modulators and proteins with immune and/or wound-healing properties. We conclude that postpartum cervical repair involves a rapid and tightly regulated balance between a host of biological factors, notably between anti- and pro-inflammatory factors, executed by the M1 and M2 macrophage cells, as revealed by proteomics and verified by confocal immunofluorescence. Future studies will assess the suitability of some of the key proteins identified in this study as potential markers for determining the phase of postpartum cervical repair in obstetrical complications, such as cervical lacerations.
及时且完全的产后子宫颈组织修复对于预防产科并发症（如宫颈炎、宫颈外翻、出血、反复流产或堕胎以及早产和恶性肿瘤）是必要的。我们最近描述了产后即刻小鼠子宫颈的形态改变以及血管生成表达谱的变化。在这里，我们在前一研究的基础上使用蛋白质组学分析来描述同一时期产后 48 小时内小鼠子宫颈组织的变化。目前的蛋白质组学数据显示，几种中间丝、细胞骨架调节剂以及具有免疫和/或伤口愈合特性的蛋白质的表达存在差异。我们得出结论，产后宫颈修复涉及宿主大量生物因子之间的快速且受到严格调控的平衡，尤其是通过 M1 和 M2 巨噬细胞细胞执行的抗炎和促炎因子之间的平衡，这一点通过蛋白质组学揭示，并通过共聚焦免疫荧光验证。未来的研究将评估本研究中鉴定的一些关键蛋白质作为确定产科并发症（如宫颈裂伤）中产后宫颈修复阶段的潜在标志物的适用性。
